Sun Shanfeng, Luo Jiangzuo, Du Hang, Liu Guirong, Liu Manman, Wang Junjuan, Han Shiwen, Che Huilian
Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, The 2115 Talent Development Program of China Agricultural University, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Foods. 2022 Jun 25;11(13):1882. doi: 10.3390/foods11131882.
Shrimp allergy (SA) is pathological type 2 inflammatory immune responses against harmless shrimp protein allergen, which is caused by complex interactions between dendritic cells (DCs) and other immune cells. Lipid metabolism in different DCs states are significantly changed. However, the lipid metabolism of spleen DCs in SA remain ambiguous. In this study, we established a BALB/c mouse shrimp protein extract-induced allergy model to determine the lipid profile of spleen DCs in SA, and the molecular mechanism between lipid metabolism and immune inflammation was preliminarily studied. Spleen DCs were sorted by fluorescence-activated cell sorting, and then widely targeted lipidomics and transcriptomics analysis were performed. Principal component analysis presented the lipidome alterations in SA. The transcriptomic data showed that was involved in lipid metabolism, immune system, and inflammatory signaling pathway. In the correlation analysis, the results suggested that was positively correlated with triacylglycerol (Pearson correlation coefficient = 0.917, = 0.01). The lipidomics and transcriptomics integrated pathway analysis indicated the activated metabolic conversion from triacylglycerol to 1,2-diacyl-sn-glycerol and the transmission of lipid metabolism to immune inflammation (from triacylglycerol and ceramide to ) in SA spleen DCs, and cellular experiments in vitro showed that glyceryl trioleate and C16 ceramide treatment induced immune function alteration in DCs.
虾过敏（SA）是针对无害虾蛋白过敏原的2型病理性炎症免疫反应，由树突状细胞（DCs）与其他免疫细胞之间的复杂相互作用引起。不同DCs状态下的脂质代谢发生显著变化。然而，SA中脾脏DCs的脂质代谢仍不明确。在本研究中，我们建立了BALB/c小鼠虾蛋白提取物诱导的过敏模型，以确定SA中脾脏DCs的脂质谱，并初步研究脂质代谢与免疫炎症之间的分子机制。通过荧光激活细胞分选对脾脏DCs进行分选，然后进行广泛靶向脂质组学和转录组学分析。主成分分析呈现了SA中的脂质组改变。转录组数据表明 参与脂质代谢、免疫系统和炎症信号通路。在相关性分析中，结果表明 与三酰甘油呈正相关（皮尔逊相关系数 = 0.917， = 0.01）。脂质组学和转录组学综合通路分析表明，SA脾脏DCs中存在从三酰甘油到1,2 - 二酰基 - sn - 甘油的激活代谢转化以及脂质代谢向免疫炎症的传递（从三酰甘油和神经酰胺到 ），体外细胞实验表明，三油酸甘油酯和C16神经酰胺处理可诱导DCs免疫功能改变。
