Engineering Research Center of Bioreactor and Pharmaceutical Development, College of Life Sciences, Jilin Agricultural University, Changchun 130118, China.
Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Genes (Basel). 2021 Sep 24;12(10):1492. doi: 10.3390/genes12101492.
is widely representing one of the important oil crops around the world, which provides valuable resources of edible tubers called tiger nut. The chemical composition and high ability to produce fats emphasize the role of tiger nut in promoting oil crop productivity. However, the underlying molecular mechanism of the production and accumulation of lipids in tiger nut development still remains unclear. Here, we conducted comprehensive transcriptomics and lipidomics analyses at different developmental stages of tuber in . Lipidomic analyses confirmed that the accumulation of lipids including glycolipids, phospholipids, and glycerides were significantly enriched during tuber development from early to mature stage. The proportion of phosphatidylcholines (PC) declined during all stages and phosphatidyl ethanolamine (PE) was significantly declined in early and middle stages. These findings implied that PC is actively involved in triacylglycerol (TAG) biosynthesis during the tubers development, whereas PE may participate in TAG metabolism during early and middle stages. Comparative transcriptomics analyses indicated several genomic and metabolic pathways associated with lipid metabolism during tuber development in tiger nut. The Pearson correlation analysis showed that TAG synthesis in different developmental stages was attributed to 37 candidate transcripts including . The up-regulation of diacylglycerol (DAG) and oil content in yeast, resulted from the inducible expression of exogenous confirmed the central role of this candidate gene in lipid metabolism. Our results demonstrated the foundation of an integrative metabolic model for understanding the molecular mechanism of tuber development in tiger nut, in which lipid biosynthesis plays a central role.
是一种广泛种植的重要油料作物，其可食用的块茎为虎坚果，提供了宝贵的资源。其化学成分和产生脂肪的高能力强调了虎坚果在提高油料作物生产力方面的作用。然而，虎坚果在发育过程中产生和积累脂质的潜在分子机制仍不清楚。在这里，我们在 不同的块茎发育阶段进行了全面的转录组学和脂质组学分析。脂质组学分析证实，从早期到成熟阶段，包括糖脂、磷脂和甘油酯在内的脂质积累在块茎发育过程中显著富集。所有阶段的磷脂酰胆碱（PC）比例下降，早期和中期的磷脂酰乙醇胺（PE）显著下降。这些发现表明，在块茎发育过程中，PC 积极参与三酰基甘油（TAG）的生物合成，而 PE 可能参与早期和中期的 TAG 代谢。比较转录组学分析表明，虎坚果在块茎发育过程中与脂质代谢相关的几个基因组和代谢途径。Pearson 相关性分析表明，不同发育阶段的 TAG 合成归因于 37 个候选转录本，包括 。外源 的诱导表达导致酵母中二酰基甘油（DAG）和油含量的上调，证实了该候选基因在脂质代谢中的核心作用。我们的研究结果为理解虎坚果块茎发育的分子机制提供了一个综合代谢模型的基础，其中脂质生物合成起着核心作用。
