Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Int J Mol Sci. 2022 Jun 29;23(13):7231. doi: 10.3390/ijms23137231.
Lasso peptides are a subclass of ribosomally synthesized and post-translationally modified peptides (RiPPs) and feature the threaded, lariat knot-like topology. The basic post-translational modifications (PTMs) of lasso peptide contain two steps, including the leader peptide removal of the ribosome-derived linear precursor peptide by an ATP-dependent cysteine protease, and the macrolactam cyclization by an ATP-dependent macrolactam synthetase. Recently, advanced bioinformatic tools combined with genome mining have paved the way to uncover a rapidly growing number of lasso peptides as well as a series of PTMs other than the general class-defining processes. Despite abundant reviews focusing on lasso peptide discoveries, structures, properties, and physiological functionalities, few summaries concerned their unique PTMs. In this review, we summarized all the unique PTMs of lasso peptides uncovered to date, shedding light on the related investigations in the future.
套索肽是核糖体合成和翻译后修饰肽 (RiPPs) 的一个子类，具有穿线、套索结状拓扑结构。套索肽的基本翻译后修饰 (PTM) 包含两个步骤，包括核糖体衍生的线性前体肽的 leader 肽去除，以及由 ATP 依赖性大环内酯合酶进行的大环内酯环化。最近，先进的生物信息学工具与基因组挖掘相结合，为发现越来越多的套索肽以及一系列除了一般定义过程之外的 PTMs 铺平了道路。尽管有大量的评论集中在套索肽的发现、结构、性质和生理功能上，但很少有综述关注它们独特的 PTMs。在这篇综述中，我们总结了迄今为止发现的所有套索肽的独特 PTMs，为未来的相关研究提供了启示。
