Department of Otolaryngology, Head and Neck Oncology, Medical University of Lodz, 90-419 Lodz, Poland.
Department of Clinical Genetics, Medical University of Lodz, 90-419 Lodz, Poland.
Int J Mol Sci. 2022 Jul 1;23(13):7345. doi: 10.3390/ijms23137345.
Studies have shown differences in receptor expression in patients with chronic rhinosinusitis (CRS) compared to healthy controls. Known agonists of stimulate epithelial cells, leading to robust intracellular nitric oxide (NO) production, which damages bacterial membranes, enzymes, and DNA, but also increases ciliary beat frequency. In this study we examined, using qRT-PCR, the expression of receptor in nasal polyps (NP) of patients with CRS (N = 107) and in inferior turbinate mucosa (ITM) of patients with CRS and controls (N = 39), and confronted it with clinical features and the severity of the disease. The expression was shown in 43 (50.00%) samples of ITM in the study group (N = 107), in 28 (71.79%) in the control group (N = 39) ( = 0.037), and in 43 (46.24%) of NP. There were no differences in levels of the expression in all analyzed tissues. Patients who rated their symptoms at 0-3 showed higher expression in ITM in comparison to the patients with 8-10 points on the VAS scale ( = 0.020). A noticeable, however not significant, correlation between the expression in ITM and the Lund-Mackay CT score was shown ( = 0.068; R = -0.28). Patients with coexisting asthma had significantly higher receptor expression in the NP ( = 0.012). Our study is the first to confirm the presence of the receptor in NP. Expression of the receptor is reduced in the sinonasal mucosa in patients with more advanced CRS with NP.
研究表明，与健康对照组相比，慢性鼻-鼻窦炎（CRS）患者的 受体表达存在差异。已知 受体的激动剂可刺激上皮细胞，导致强烈的细胞内一氧化氮（NO）产生，从而破坏细菌膜、酶和 DNA，但也会增加纤毛摆动频率。在这项研究中，我们使用 qRT-PCR 检查了 CRS 患者的鼻息肉（NP）（N=107）和 CRS 患者和对照组的下鼻甲黏膜（ITM）（N=39）中 受体的表达，并将其与临床特征和疾病严重程度进行了比较。在研究组的 107 个样本中，有 43 个（50.00%）的 ITM 表达了 受体，在对照组的 39 个样本中，有 28 个（71.79%）表达了 受体（=0.037），NP 中有 43 个（46.24%）表达了 受体。在所有分析的组织中，表达水平没有差异。在 VAS 量表上评分为 0-3 的患者的 ITM 中的 表达水平明显高于评分为 8-10 分的患者（=0.020）。然而，在 ITM 中的 表达与 Lund-Mackay CT 评分之间显示出显著但无统计学意义的相关性（=0.068；R=-0.28）。同时患有哮喘的患者的 NP 中的受体表达明显更高（=0.012）。我们的研究首次证实了 受体在 NP 中的存在。在伴有 NP 的更严重 CRS 患者的鼻-鼻窦黏膜中， 受体的表达减少。
