College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
Int J Mol Sci. 2022 Jul 4;23(13):7427. doi: 10.3390/ijms23137427.
Ovate family proteins (OFPs) are valued as a family of transcription factors that are unique to plants, and they play a pluripotent regulatory role in plant growth and development, including secondary-cell-wall synthesis, DNA repair, gibberellin synthesis, and other biological processes, via their interaction with TALE family proteins. In this study, CHIP-SEQ was used to detect the potential target genes of AtOFP1 and its signal-regulation pathways. On the other hand, Y2H and BIFC were employed to prove that AtOFP1 can participate in ABA signal transduction by interacting with one of the TALE family protein called AtKNAT3. ABA response genes are not only significantly upregulated in the OE line, but they also show hypersensitivity to ABA in terms of seed germination and early seedling root elongation. In addition, the AtOFP1-regulated target genes are mainly mitochondrial membranes that are involved in the oxidative-phosphorylation pathway. Further qRT-PCR results showed that the inefficient splicing of the respiratory complex I subunit genes and may lead to ROS accumulation in OE lines. In conclusion, we speculated that the overexpression of AtOFP1 may cause the ABA hypersensitivity response by increasing the intracellular ROS content generated from damage to the intima systems of mitochondria.
卵形家族蛋白（OFPs）是一类植物特有的转录因子，因其与 TALE 家族蛋白的相互作用，在植物生长发育的多个方面发挥着多功能的调控作用，包括次生细胞壁合成、DNA 修复、赤霉素合成和其他生物学过程。在本研究中，CHIP-SEQ 被用于检测 AtOFP1 的潜在靶基因及其信号调控途径。另一方面，Y2H 和 BIFC 被用来证明 AtOFP1 可以通过与 TALE 家族蛋白之一 AtKNAT3 相互作用参与 ABA 信号转导。ABA 响应基因不仅在 OE 系中显著上调，而且在种子萌发和早期幼苗根伸长方面对 ABA 表现出超敏反应。此外，AtOFP1 调节的靶基因主要是涉及氧化磷酸化途径的线粒体膜。进一步的 qRT-PCR 结果表明，呼吸复合物 I 亚基基因 和 的无效剪接可能导致 OE 系中 ROS 的积累。总之，我们推测 AtOFP1 的过表达可能通过增加来自线粒体内膜系统损伤的细胞内 ROS 含量引起 ABA 超敏反应。
