Laboratory of Epidemiology and Research in Health Sciences, Faculty of Medicine and Pharmacy, Dental Medicine University of Sidi Mohammed Ben Abdellah, Fez 30070, Morocco.
Laboratory of Biotechnology, Environment, Agri-Food, and Health (LBEAS), Faculty of Sciences, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco.
Molecules. 2022 Jun 21;27(13):3973. doi: 10.3390/molecules27133973.
Ethnobotanical studies have reported the traditional medicinal uses of Acacia senegal (L.) Willd. and Argania spinosa (L.) Skeels against kidney stone formation and other chronic kidney diseases. The present work is undertaken to study the litholytic activity and the inhibiting activity of calcium oxalate crystallization by bioactive compounds identified in Argania spinosa (L.) Skeels press-cake (residue of Argan oil) and in Acacia senegal (L.) Willd. The litholytic activity was studied in vitro on cystine and uric acid stones using a porous bag and an Erlenmeyer glass. The study of the inhibiting activity of calcium oxalate crystallization, was based on temporal measurements of the optical density, registered at a 620 nm wavelength for 30 min using an ultraviolet−visible spectrophotometer. The silylation method was performed to identify phytochemicals, followed by gas chromatography coupled with mass spectrophotometry (GC/MS) analysis. The results show significant litholytic activity of Argania Spinosa press-cake hydro-ethanolic extract on uric acid and cystine stones, respectively, with dissolution rates (DR) of 86.38% and 60.42% versus 3.23% and 9.48% for the hydro-ethanolic extract of Acacia senegal exudate. Furthermore, the percentages of nucleation inhibition are 83.78% and 43.77% (p ˂ 0.05) for Argania spinosa and Acacia senegal, respectively. The results point to the detection of 17 phytochemicals in Argania spinosa press-cake extract, the majority of which are phenolic acids and have potent anti-urolithiatic action.
民族植物学研究报告了金合欢属（L.）Willd. 和阿甘属（L.）Skeels 对肾结石形成和其他慢性肾病的传统药用用途。本工作旨在研究从阿甘属（L.）Skeels 压榨饼（阿甘油残渣）和金合欢属（L.）Willd. 中鉴定的生物活性化合物的溶石活性和抑制草酸钙结晶的活性。在体外使用多孔袋和摇瓶研究胱氨酸和尿酸结石的溶石活性。草酸钙结晶抑制活性的研究基于在 30 分钟内使用紫外可见分光光度计在 620nm 波长处记录的光密度的时间测量。进行硅烷化方法以鉴定植物化学物质，然后进行气相色谱-质谱联用（GC/MS）分析。结果表明，阿甘属（L.）Spinosa 压榨饼水醇提取物对尿酸结石和胱氨酸结石具有显著的溶石活性，溶解率（DR）分别为 86.38%和 60.42%，而金合欢属（L.）Senegal 渗出物的水醇提取物分别为 3.23%和 9.48%。此外，阿甘属（L.）Spinosa 和金合欢属（L.）Senegal 的成核抑制率分别为 83.78%和 43.77%（p ˂ 0.05）。结果表明，在阿甘属（L.）Spinosa 压榨饼提取物中检测到 17 种植物化学物质，其中大多数为酚酸，具有很强的抗尿石作用。
