College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China.
College of Agriculture and Bioengineering, Heze University, Heze 274000, China.
Molecules. 2022 Jun 25;27(13):4089. doi: 10.3390/molecules27134089.
This work aims to study the antioxidant interactions between S-allyl-L-cysteine (SAC) and six natural polyphenols (quercetin, caffeic acid, sinapic acid, catechin, ferulic acid, and 3,4-dihydroxybenzoic acid) through the measurement of free-radical-scavenging activity of 1,1-diphenyl- 2-picryl-hydrazyl (DPPH), the radical-cation-scavenging activity of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and reducing power. Among the six natural polyphenols, caffeic acid showed the strongest synergistic effect with SAC according to DPPH and reducing power assays. Further investigations based on the results of interaction index and isobologram analysis showed that the antioxidant activity (DPPH, ABTS, and reducing power) of the combination of caffeic acid with SAC presented an increase with the raising of their individual concentrations in their mixture and along with a dose-response manner. The best synergistic effect between caffeic acid and SAC based on DPPH, ABTS, and reducing power assays were observed at the ratio of 1:20, 1:35, and 1:70, respectively. The excellent synergic antioxidant activity of the combination of caffeic acid with SAC in our study suggests SAC has a more broad and effective application prospects in food field.
本研究旨在通过 1,1-二苯基-2-苦基肼基（DPPH）自由基清除活性、2,2-连氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基阳离子清除活性和还原力来研究 S-烯丙基-L-半胱氨酸（SAC）与六种天然多酚（槲皮素、咖啡酸、芥子酸、儿茶素、阿魏酸和 3,4-二羟基苯甲酸）之间的抗氧化相互作用。在这六种天然多酚中，根据 DPPH 和还原力测定，咖啡酸与 SAC 表现出最强的协同作用。基于相互作用指数和等效应线分析的进一步研究表明，咖啡酸与 SAC 组合的抗氧化活性（DPPH、ABTS 和还原力）随着混合物中各自浓度的升高而增加，并呈现剂量反应关系。根据 DPPH、ABTS 和还原力测定，咖啡酸与 SAC 的最佳协同作用比例分别为 1:20、1:35 和 1:70。本研究中咖啡酸与 SAC 组合具有优异的协同抗氧化活性，表明 SAC 在食品领域具有更广泛和有效的应用前景。
