College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Oxid Med Cell Longev. 2021 Jul 9;2021:9996040. doi: 10.1155/2021/9996040. eCollection 2021.
Araloside A is a pentacyclic triterpenoid saponin, and L-ascorbic acid is a globally recognized antioxidant. In this study, coadministered araloside A and L-ascorbic acid were found to have a strong synergistic antioxidant effect, and correlations between cellular antioxidant indexes and free radical scavenging ability were found. Individual and combined pretreatment with araloside A and L-ascorbic acid increased both cell viability and antioxidant enzyme activity and inhibited the release of lactate dehydrogenase (LDH); the accumulation of malondialdehyde (MDA), lipid peroxidation (LPO) products, and HO; and the production of intracellular reactive oxygen species (ROS), protein carbonyls, and 8-hydroxy-2-deoxy guanosine (8-OHdG). Free radical scavenging ability was positively correlated with superoxide dismutase (SOD) and catalase (CAT) activity, the glutathione (GSH)/oxidized glutathione (GSSG) ratio, and total antioxidant capacity (T-AOC). Our study is the first investigation of araloside A and L-ascorbic acid coadministration for the treatment of diseases caused by oxidative stress. The synergistic antioxidant effects of araloside A and L-ascorbic acid support their potential as functional food ingredients for the elimination of oxidative stress-induced adverse reactions.
梓醇 A 是一种五环三萜皂苷，L-抗坏血酸是一种全球公认的抗氧化剂。在这项研究中，发现梓醇 A 和 L-抗坏血酸联合使用具有很强的协同抗氧化作用，并且发现细胞抗氧化指标与自由基清除能力之间存在相关性。梓醇 A 和 L-抗坏血酸的单独和联合预处理均提高了细胞活力和抗氧化酶活性，并抑制了乳酸脱氢酶（LDH）的释放；丙二醛（MDA）、脂质过氧化（LPO）产物和羟基（HO）的积累；以及细胞内活性氧（ROS）、蛋白质羰基和 8-羟基-2-脱氧鸟苷（8-OHdG）的产生。自由基清除能力与超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、谷胱甘肽（GSH）/氧化谷胱甘肽（GSSG）比值和总抗氧化能力（T-AOC）呈正相关。本研究首次探讨了梓醇 A 和 L-抗坏血酸联合用药治疗氧化应激引起的疾病。梓醇 A 和 L-抗坏血酸的协同抗氧化作用支持它们作为功能性食品成分的潜力，可消除氧化应激引起的不良反应。
