Engineering College, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161006, China.
Molecules. 2022 Jun 25;27(13):4091. doi: 10.3390/molecules27134091.
Rice blast is a serious threat to rice yield. Breeding disease-resistant varieties is one of the most economical and effective ways to prevent damage from rice blast. The traditional identification of resistant rice seeds has some shortcoming, such as long possession time, high cost and complex operation. The purpose of this study was to develop an optimal prediction model for determining resistant rice seeds using Ranman spectroscopy. First, the support vector machine (SVM), BP neural network (BP) and probabilistic neural network (PNN) models were initially established on the original spectral data. Second, due to the recognition accuracy of the Raw-SVM model, the running time was fast. The support vector machine model was selected for optimization, and four improved support vector machine models (ABC-SVM (artificial bee colony algorithm, ABC), IABC-SVM (improving the artificial bee colony algorithm, IABC), GSA-SVM (gravity search algorithm, GSA) and GWO-SVM (gray wolf algorithm, GWO)) were used to identify resistant rice seeds. The difference in modeling accuracy and running time between the improved support vector machine model established in feature wavelengths and full wavelengths (200-3202 cm) was compared. Finally, five spectral preproccessing algorithms, Savitzky-Golay 1-Der (SGD), Savitzky-Golay Smoothing (SGS), baseline (Base), multivariate scatter correction (MSC) and standard normal variable (SNV), were used to preprocess the original spectra. The random forest algorithm (RF) was used to extract the characteristic wavelengths. After different spectral preproccessing algorithms and the RF feature extraction, the improved support vector machine models were established. The results show that the recognition accuracy of the optimal IABC-SVM model based on the original data was 71%. Among the five spectral preproccessing algorithms, the SNV algorithm's accuracy was the best. The accuracy of the test set in the IABC-SVM model was 100%, and the running time was 13 s. After SNV algorithms and the RF feature extraction, the classification accuracy of the IABC-SVM model did not decrease, and the running time was shortened to 9 s. This demonstrates the feasibility and effectiveness of IABC in SVM parameter optimization, with higher prediction accuracy and better stability. Therefore, the improved support vector machine model based on Ranman spectroscopy can be applied to the fast and non-destructive identification of resistant rice seeds.
稻瘟病是水稻产量的严重威胁。培育抗病品种是防止稻瘟病造成损害的最经济、最有效的方法之一。传统的抗病种子鉴定存在一些缺点,如占用时间长、成本高、操作复杂。本研究旨在利用 Raman 光谱开发一种最佳的预测模型来确定抗病水稻种子。首先,在原始光谱数据上初步建立支持向量机(SVM)、BP 神经网络(BP)和概率神经网络(PNN)模型。其次,由于 Raw-SVM 模型的识别准确率高,运行时间较快,选择支持向量机模型进行优化,建立了四个改进的支持向量机模型(ABC-SVM(人工蜂群算法,ABC)、IABC-SVM(改进的人工蜂群算法,IABC)、GSA-SVM(引力搜索算法,GSA)和 GWO-SVM(灰狼算法,GWO))来识别抗病水稻种子。比较了在特征波长和全波长(200-3202 cm)下建立的改进支持向量机模型的建模精度和运行时间的差异。最后,使用 Savitzky-Golay 1-Der(SGD)、Savitzky-Golay Smoothing(SGS)、基线(Base)、多元散射校正(MSC)和标准正态变量(SNV)五种光谱预处理算法对原始光谱进行预处理,随机森林算法(RF)提取特征波长。在不同的光谱预处理算法和 RF 特征提取后,建立了改进的支持向量机模型。结果表明,基于原始数据的最优 IABC-SVM 模型的识别准确率为 71%。在五种光谱预处理算法中,SNV 算法的准确率最高。IABC-SVM 模型的测试集准确率为 100%,运行时间为 13 s。在经过 SNV 算法和 RF 特征提取后,IABC-SVM 模型的分类准确率没有降低,运行时间缩短到 9 s。这表明 IABC 在 SVM 参数优化中具有可行性和有效性,具有更高的预测精度和更好的稳定性。因此,基于 Raman 光谱的改进支持向量机模型可应用于抗病水稻种子的快速、无损识别。