根皮素和去甲根皮素处理 T-淋巴母细胞白血病 CEM-SS 细胞后的超微结构和形态学效应

Ultrastructural and Morphological Effects in T-Lymphoblastic Leukemia CEM-SS Cells Following Treatment with Nordamnacanthal and Damnacanthal from Roots of .

机构信息

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia.

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia.

出版信息

Molecules. 2022 Jun 28;27(13):4136. doi: 10.3390/molecules27134136.

DOI:10.3390/molecules27134136

PMID:35807381

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9268620/

Abstract

BACKGROUND

(family Rubiaceae), locally known as 'mengkudu kecil', has been used by the Malays for medicinal purposes. Anthraquinones isolated from the roots of , namely nordamnacanthal and damnacanthal, have been widely reported to exhibit anticancer and antioxidant properties in various cancer models in vitro and in vivo.

AIM

This study analyzed the morphological and ultrastructural effects of damnacanthal and nordamnacanthal on T-lymphoblastic leukemia CEM-SS cells.

METHOD

Light microscopy, Giemsa staining, Wright's staining, scanning electron microscopy, and transmission electron microscopy were carried out to determine apoptosis, necrosis, and ultrastructural changes that occurred within the cells.

RESULTS

The outcomes showed that these compounds induced cell death by apoptosis and necrosis, specifically at higher doses of 10 and 30 μg/mL. Condensation and fragmentation of the nuclear chromatin, which further separated into small, membrane-bound vesicles known as apoptotic bodies, were observed in the nuclei and cytoplasm. The plasma membranes and cytoskeletons also showed marked morphological changes upon treatment with damnacanthal and nordamnacanthal, indicating apoptosis.

CONCLUSION

Therefore, we report that damnacanthal and nordamnacanthal exhibit anticancer properties by inducing apoptosis and necrosis in CEM-SS cells, and they have potential as a drug for the treatment of T-lymphoblastic leukemia.

摘要

背景

（茜草科Rubiaceae），当地俗称“mengkudu kecil”，马来人一直将其用于药用目的。从该植物根部分离出的蒽醌类化合物，即 nordamnacanthal 和 damnacanthal，已被广泛报道在体外和体内的各种癌症模型中具有抗癌和抗氧化特性。

目的

本研究分析 damnacanthal 和 nordamnacanthal 对 T 淋巴细胞白血病 CEM-SS 细胞的形态和超微结构影响。

方法

通过光镜、吉姆萨染色、瑞氏染色、扫描电子显微镜和透射电子显微镜观察细胞凋亡、坏死以及细胞内发生的超微结构变化。

结果

结果表明，这些化合物通过凋亡和坏死诱导细胞死亡，特别是在更高剂量 10 和 30 μg/mL 时。细胞核和细胞质中观察到核染色质的浓缩和碎裂，进一步分离成称为凋亡小体的小的、膜结合的囊泡。用 damnacanthal 和 nordamnacanthal 处理后，质膜和细胞骨架也显示出明显的形态变化，表明发生了凋亡。