Juškytė Ana Dovilė, Mažeikienė Ingrida, Stanys Vidmantas
Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Kaunas str. 30, 54333 Babtai, Lithuania.
Plants (Basel). 2022 Jun 21;11(13):1635. doi: 10.3390/plants11131635.
Blackcurrant reversion virus (BRV) is the most destructive currant-infecting and mite-transmitted pathogen from the genus . In this work, BRV transmission in the system ex vitro- in vitro was applied for the first time. Triple infection of BRV identified in blackcurrant cv. Gojai was used for phylogenetic analysis and inoculation assay. Transmission of BRV was successful due to its stability in the inoculum for up to 8 days at 4 °C; all BRV isolates were infectious. Our suggested inoculation method through roots was applied in six spp. genotypes with 100.0% reliability, and the expression levels of defence-related gene to biotic stress was observed. The prevalence of the virus in microshoots after 2-14 days post-inoculation (dpi) was established by PCR. In resistant genotypes, the BRV was identified up to 8 dpi; meanwhile, infection remained constant in susceptible genotypes. We established that BRV transmission under controlled conditions depends on the inoculum quality, post-inoculation cultivation temperature, and host-plant susceptibility to pathogen. This in vitro inoculation method opens possibilities to reveal the resistance mechanisms or response pathways to BRV and can be used for the selection of resistant spp. in breeding programs.
黑加仑逆转病毒（BRV）是该属中最具破坏性的感染黑加仑且由螨传播的病原体。在这项工作中，首次应用了BRV在离体-离体系统中的传播。在黑加仑品种Gojai中鉴定出的BRV三重感染用于系统发育分析和接种试验。BRV的传播取得成功，这是因为其接种物在4℃下长达8天具有稳定性；所有BRV分离株均具有感染性。我们建议的通过根部接种的方法在6种基因型中应用，可靠性达100.0%，并观察了与生物胁迫相关的防御基因的表达水平。接种后2至14天（dpi）通过PCR确定微芽中病毒的流行情况。在抗性基因型中，直至接种后8天可鉴定出BRV；同时，在易感基因型中感染保持稳定。我们确定，在受控条件下BRV的传播取决于接种物质量、接种后培养温度以及寄主植物对病原体的易感性。这种离体接种方法为揭示对BRV的抗性机制或反应途径提供了可能，可用于在育种计划中选择抗性品种。
