Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Kaunas str. 30, 54333 Babtai, Lithuania.
Int J Mol Sci. 2022 Aug 24;23(17):9560. doi: 10.3390/ijms23179560.
The most damaging pathogen in blackcurrant plantations is mite-transmitted blackcurrant reversion virus (BRV). Some species have an encoded genetic resistance to BRV. We performed RNA sequencing analysis of BRV-resistant blackcurrant cv. Aldoniai to evaluate the molecular mechanisms related to the BRV infection response. The RNA of virus-inoculated and mock-inoculated microshoots was sequenced, and the transcriptional changes at 2- and 4-days post inoculation (dpi) were analyzed. The accumulation and expression of BRV RNA1 were detected in infected plants. In total, 159,701 transcripts were obtained and 30.7% were unigenes, annotated in 7 databases. More than 25,000 differentially expressed genes (DEGs) according to FPKM were upregulated or downregulated. We observed 221 and 850 DEGs at 2 and 4 dpi, respectively, in BRV-infected microshoots related to the stress response. The proportion of upregulated DEGs at 4 dpi was about 3.5 times higher than at 2 dpi. Pathways of the virus defense response were activated, and key candidate genes were identified. The phenylpropanoid and the cutin, suberine, and wax biosynthesis pathways were activated in infected plants. Our comparative de novo analysis of the transcriptome provides clues not only for understanding the molecular BRV resistance mechanisms but also for breeding BRV-tolerant genotypes.
黑醋栗种植园中最具破坏性的病原体是螨传黑醋栗返祖病毒（BRV）。有些品种对 BRV 具有编码的遗传抗性。我们对 BRV 抗性黑醋栗品种 Aldoniai 进行了 RNA 测序分析，以评估与 BRV 感染反应相关的分子机制。对接种病毒和模拟接种的微型插条的 RNA 进行了测序，并分析了接种后 2 天和 4 天（dpi）的转录变化。检测了感染植物中 BRV RNA1 的积累和表达。总共获得了 159701 个转录本，其中 30.7%为 unigenes，在 7 个数据库中进行了注释。根据 FPKM 上调或下调的差异表达基因（DEG）超过 25000 个。我们在 BRV 感染的微型插条中观察到 2 和 4 dpi 分别有 221 和 850 个与应激反应相关的 DEG。4 dpi 时上调 DEG 的比例比 2 dpi 时高约 3.5 倍。病毒防御反应途径被激活，并鉴定了关键候选基因。感染植物中激活了苯丙烷和角质、栓皮和蜡生物合成途径。我们对转录组的比较从头分析不仅为理解分子 BRV 抗性机制提供了线索，而且为培育 BRV 耐受基因型提供了线索。
