Kibkalo Ilya
N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia.
Plants (Basel). 2022 Jun 21;11(13):1640. doi: 10.3390/plants11131640.
The existing standardized methods for assessing the quality of marketable grain do not always meet the requirements of the breeding, such as the method's rapidity, sufficiency of the minimum amount of experimental material, the minimal modifying influence of the external environment on the degree of expression of a criterion, and genetic determination and heritability of the latter. One of the methods that meets these requirements is the sediment volume test. The present study offers an analysis and examples of methodological developments in relation to the assessment of winter bread wheat grain in arid regions of cultivation, as well as of winter triticale. The fluorescent probing method was used as an example for demonstrating the prospects for assessing the swelling of ground grain products of both bread and durum wheat, and for such crops with a less-strong complex of storage polymers as triticale, rye, and millet. A two-stage sedimentation procedure that allows a successful differentiation of samples has been developed for sorghum and maize grain. It is presented here alongside with methodological works on wheat from different countries of the world. Examples of the proven high reproducibility of the sediment volume test in the offspring, and its genetic determination are provided. In general, the data obtained and the material accumulated by various researchers indicate that a modification of the sedimentation method, correctly chosen for specific goals and objectives, solves the problem of assessing grain quality in breeding samples starting from early progenies. All these circumstances make the sedimentation testing the leading or most promising method for assessing grain quality when breeding of a broad range of grain crops is carried out.
现有的用于评估适销谷物质量的标准化方法并不总是能满足育种的要求,比如该方法的快速性、所需实验材料最小量的充足性、外部环境对标准表达程度的最小修饰影响,以及后者的遗传决定性和遗传力。沉降体积测试是符合这些要求的方法之一。本研究对干旱种植区冬小麦籽粒以及冬小黑麦的评估方法进展进行了分析并给出了实例。以荧光探测法为例,展示了评估面包小麦和硬粒小麦磨碎谷物产品膨胀情况的前景,以及对诸如小黑麦、黑麦和黍等储存聚合物复合物较弱的作物的评估前景。针对高粱和玉米籽粒,开发了一种能成功区分样品的两阶段沉降程序。本文还展示了来自世界不同国家关于小麦的方法学研究成果。提供了沉降体积测试在后代中具有高重现性及其遗传决定性的实例。总体而言,不同研究人员获得的数据和积累的材料表明,针对特定目标正确选择的沉降方法的改进,解决了从早期后代开始评估育种样品中籽粒质量的问题。所有这些情况使得沉降测试成为开展多种谷物作物育种时评估籽粒质量的主导或最有前景的方法。