Radchenko Evgeny E, Anisimova Irina N, Ryazanova Maria K, Kibkalo Ilya A, Alpatieva Natalia V
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 St. Petersburg, Russia.
Plants (Basel). 2024 Jan 31;13(3):425. doi: 10.3390/plants13030425.
Eight lines of grain sorghum [ (L.) Moench], which can be used as a promising source material in heterotic hybrid breeding as pollen fertility restorers and donors of resistance to the greenbug ( Rondani), are characterized. The new restorer lines (R-lines) were developed by crossing the maternal sterile line Nizkorosloe 81s (CMS A1) with two lines selected from the grain sorghum collection accessions VIR-928 and VIR-929 as the paternal forms. The R-lines were genotyped using PCR markers, and also characterized by height, duration of the seedling-flowering period, and some of the technological properties of flour. With the use of microsatellite markers linked to the genes and by hybridological analysis, it was shown that the new lines carry the dominant allele of the gene . The PCoA analysis demonstrated clear differences of each R-line from the parents. The genotypes of the new lines and their parental forms for the locus were confirmed by applying three allele-specific codominant CAPS markers which detected SNPs in the candidate gene. All new lines were highly fertile, as demonstrated by cytological analysis of acetocarmine-stained pollen preparations. A high resistance to the greenbug was demonstrated for each new R-line both in the laboratory and field conditions against a severe aphid infestation. Grain quality parameters such as protein content and dough rheological properties varied widely and were quite satisfactory in some R-lines. Characteristics common to all eight sorghum lines studied, such as the ability to restore pollen fertility in the F generation, good pollen quality, greenbug resistance, early ripening, spreading panicle, and low stature, allow us to recommend them for producing commercial F hybrids with satisfactory grain quality for both fodder and food purposes.
对8个粒用高粱品系[ (L.) Moench]进行了特征描述，这些品系可作为杂种优势杂交育种中很有前景的种质材料，用作花粉育性恢复系和抗麦二叉蚜（Rondani）的供体。新的恢复系（R系）是通过将母本不育系Nizkorosloe 81s（CMS A1）与从粒用高粱收集种质VIR - 928和VIR - 929中选出的两个品系作为父本形式杂交培育而成。利用PCR标记对R系进行基因分型，并对其株高、苗期至开花期的持续时间以及面粉的一些工艺特性进行了表征。通过使用与 基因连锁的微卫星标记和杂交分析，表明新系携带 基因的显性等位基因。主成分分析（PCoA）表明每个R系与其亲本存在明显差异。通过应用三个等位基因特异性共显性CAPS标记，检测候选 基因中的单核苷酸多态性（SNP），证实了新系及其亲本形式在 位点的基因型。通过对醋酸洋红染色花粉制片的细胞学分析表明，所有新系均高度可育。在实验室和田间条件下，每个新的R系对严重的蚜虫侵染均表现出对麦二叉蚜的高抗性。蛋白质含量和面团流变学特性等籽粒品质参数差异很大，在一些R系中相当令人满意。所研究的所有8个高粱品系共有的特征，如在F代恢复花粉育性的能力、良好的花粉质量、抗麦二叉蚜、早熟、散穗和矮株型，使我们推荐它们用于生产具有令人满意的籽粒品质的商业F1杂种，用于饲料和食品目的。
