Mrkvová Michaela, Hančinský Richard, Predajňa Lukáš, Alaxin Peter, Achs Adam, Tomašechová Jana, Šoltys Katarína, Mihálik Daniel, Olmos Antonio, Ruiz-García Ana Belén, Glasa Miroslav
Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Nám. J. Herdu 2, 91701 Trnava, Slovakia.
National Agricultural and Food Centre, Research Institute of Plant Production, Bratislavská cesta 122, 92168 Piešt'any, Slovakia.
Plants (Basel). 2022 Jun 23;11(13):1665. doi: 10.3390/plants11131665.
Cucumber mosaic virus (CMV; ) is an omnipresent virus characterized by a large host range and high genetic variability. Using high-throughput sequencing, we have characterized near complete genomes of 14 Slovak CMV variants from different plant hosts. Of these, three variants originated from the species (oilseed poppy, common poppy and great celandine), previously poorly described as CMV natural hosts. Based on a BLAST search and phylogenetic analysis, the Slovak CMV isolates can be divided into two genetically different Groups, Ia and II, respectively. The SL50V variant, characterized by a divergent RNA2 sequence, potentially represents a reassortant variant. In four samples (T101, SL50V, CP2, MVU2-21), the presence of satellite CMV RNA was identified along with CMV. Although mechanically transmitted to experimental cucumber plants, the role of satellite RNA in the symptomatology observed could not be established due to a complex infection of original hosts with different viruses.
黄瓜花叶病毒（CMV）是一种广泛存在的病毒，其特点是宿主范围广且遗传变异性高。我们利用高通量测序技术，对来自不同植物宿主的14个斯洛伐克CMV变体的近乎完整的基因组进行了特征分析。其中，有三个变体源自罂粟属物种（油籽罂粟、虞美人、白屈菜），此前这些物种作为CMV自然宿主的相关描述较少。基于BLAST搜索和系统发育分析，斯洛伐克CMV分离株可分别分为两个遗传上不同的组，即Ia组和II组。以不同的RNA2序列为特征的SL50V变体可能代表一种重配变体。在四个样本（T101、SL50V、CP2、MVU2 - 21）中，除了CMV外，还鉴定出了卫星CMV RNA的存在。尽管通过机械接种将其传播到了实验黄瓜植株上，但由于原始宿主受到不同病毒的复杂感染，无法确定卫星RNA在观察到的症状学中所起的作用。
