Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Nutrients. 2022 Jun 22;14(13):2584. doi: 10.3390/nu14132584.
Probiotics, active microorganisms benefiting human health, currently serve as nutritional supplements and clinical treatments. Periodontitis, a chronic infectious oral disease caused by (), activates the host immune response to release numerous proinflammatory cytokines. Here, we aimed to clarify () LVBH107 probiotic effects based on the inhibition of activities while also evaluating the effectiveness of an in vitro lipopolysaccharide-stimulated RAW 264.7 cell-based inflammation mode. LVBH107 survived at acid, bile salts, lysozyme, and hydrogen peroxide conditions, auto-aggregated and co-aggregated with , exhibited strong hydrophobicity and electrostatic action, and strongly adhered to gingival epithelial and HT-29 cells (thus exhibiting oral tissue adherence and colonization abilities). Moreover, LVBH107 exhibited sensitivity to antibiotics erythromycin, doxycycline, minocycline, ampicillin, and others (thus indicating it lacked antibiotic resistance plasmids), effectively inhibited biofilm formation and inflammation (in vitro inflammation model), reduced the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) and inflammatory mediators (NO and PGE), and decreased the expression levels of inflammation related genes. Thus, LVBH107 holds promise as a probiotic that can inhibit biofilm formation and exert anti-inflammatory activity to maintain oral health.
益生菌是有益于人类健康的活性微生物，目前既作为营养补充剂，又作为临床治疗方法。由（）引起的慢性感染性口腔疾病牙周炎会激活宿主免疫反应，释放大量促炎细胞因子。在这里，我们旨在基于抑制（）活性来阐明（）LVBH107 益生菌的功效，同时评估体外（）脂多糖刺激 RAW 264.7 细胞炎症模型的有效性。LVBH107 能在酸性、胆汁盐、溶菌酶和过氧化氢条件下存活，能自动聚集和与（）共聚，表现出较强的疏水性和静电力，并能强烈黏附于牙龈上皮细胞和 HT-29 细胞（因此表现出口腔组织黏附和定植能力）。此外，（）LVBH107 对红霉素、强力霉素、米诺环素、氨苄西林等抗生素敏感（因此表明其缺乏抗生素抗性质粒），能有效抑制（）生物膜形成和炎症（体外炎症模型），减少促炎细胞因子（TNF-α、IL-6 和 IL-1β）和炎症介质（NO 和 PGE）的分泌，并降低炎症相关基因的表达水平。因此，（）LVBH107 有望成为一种益生菌，能抑制（）生物膜形成并发挥抗炎活性，以维持口腔健康。
