Shariare Mohammad Hossain, Pinky Nusrat Jahan Khan, Abedin Joynal, Kazi Mohsin, Aldughaim Mohammed S, Uddin Mohammad N
Department of Pharmaceutical Sciences, North South University, Dhaka 1229, Bangladesh.
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Nanomaterials (Basel). 2022 Jun 30;12(13):2262. doi: 10.3390/nano12132262.
() is a herbaceous plant commonly found in south-east Asia. It shows significant therapeutic activities against various diseases. The objectives of this study were to evaluate hepatoprotective effects of leaf extract and also to investigate the comparative effectiveness between a liposomal preparation and a suspension of leaf extract against carbon tetrachloride (CCl)-induced liver damage.
leaf extract was characterized using a GC-MS method. A liposomal preparation of leaf extract was developed using an ethanol injection method and characterized using dynamic light scattering (DLS) and electronic microscopic systems. The hepatoprotective effects of leaf extracts and its liposomal preparation were investigated using CCl-induced liver damage in Long Evan rats.
GC-MS data showed the presence of different components (e.g., phytol) in the leaf extract. DLS and microscopic data showed that a liposomal preparation of leaf extracts was in the nano size range. In vivo study results showed that liposomal preparation and a suspension of leaf extract normalized liver biochemical parameters, enzymes and oxidative stress markers which were elevated due to CCl administration. However, a liposomal formulation of leaf extract showed significantly better hepatoprotective effects compared to a suspension of leaf extract. In addition, histopathological evaluation showed that leaf extract and its liposomal preparation treatments decreased the extent of CCl-induced liver inflammations.
Results demonstrated that leaf extract was effective against CCl-induced liver injury possibly due to the presence of components such as phytol. A liposomal preparation exhibited significantly better activity compared to a suspension, probably due to improved bioavailability and stability of the leaf extract.
（植物名称）是一种常见于东南亚的草本植物。它对多种疾病显示出显著的治疗活性。本研究的目的是评估（植物名称）叶提取物的肝保护作用，并研究叶提取物的脂质体制剂与叶提取物悬浮液对四氯化碳（CCl）诱导的肝损伤的相对有效性。
采用气相色谱 - 质谱（GC - MS）方法对叶提取物进行表征。采用乙醇注入法制备叶提取物的脂质体制剂，并使用动态光散射（DLS）和电子显微镜系统进行表征。在Long Evan大鼠中，利用CCl诱导的肝损伤研究叶提取物及其脂质体制剂的肝保护作用。
GC - MS数据显示叶提取物中存在不同成分（如叶绿醇）。DLS和显微镜数据表明叶提取物的脂质体制剂处于纳米尺寸范围。体内研究结果表明，脂质体制剂和叶提取物悬浮液使因给予CCl而升高的肝脏生化参数、酶和氧化应激标志物恢复正常。然而，叶提取物的脂质体制剂与叶提取物悬浮液相比，显示出显著更好的肝保护作用。此外，组织病理学评估表明，叶提取物及其脂质体制剂处理降低了CCl诱导的肝脏炎症程度。
结果表明，叶提取物对CCl诱导的肝损伤有效，可能是由于存在叶绿醇等成分。与悬浮液相比，脂质体制剂表现出显著更好的活性，可能是由于叶提取物的生物利用度和稳定性提高。
