Blasa Stefania, Borzenkov Mykola, Pastori Valentina, Doveri Lavinia, Pallavicini Piersandro, Chirico Giuseppe, Lecchi Marzia, Collini Maddalena
Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
Department of Medicine and Surgery, Nanomedicine Center, University of Milano-Bicocca, Via Alfred Nobel, 20854 Vedano al Lambro, Italy.
Nanomaterials (Basel). 2022 Jul 4;12(13):2304. doi: 10.3390/nano12132304.
Heating has recently been applied as an alternative to electrical stimulation to modulate excitability and to induce neuritogenesis and the expression of neuronal markers; however, a long-term functional differentiation has not been described so far. Here, we present the results obtained by a new approach for scalable thermal stimulation on the behavior of a model of dorsal root ganglion neurons, the F-11 cell line. Initially, we performed experiments of bulk stimulation in an incubator for different time intervals and temperatures, and significant differences in neurite elongation and in electrophysiological properties were observed in cultures exposed at 41.5 °C for 30 min. Thus, we exposed the cultures to the same temperature increase using a near-infrared laser to irradiate a disc of Prussian blue nanoparticles and poly-vinyl alcohol that we had adhered to the outer surface of the petri dish. In irradiated cells, neurites were significantly longer, and the electrophysiological properties (action potential firing frequency and spontaneous activity) were significantly increased compared to the control. These results show for the first time that a targeted thermal stimulation could induce morphological and functional neuronal differentiation and support the future application of this method as a strategy to modify neuronal behavior in vivo.
加热最近已被用作电刺激的替代方法，以调节兴奋性并诱导神经突生成和神经元标志物的表达；然而，迄今为止尚未描述长期功能分化情况。在此，我们展示了一种对背根神经节神经元模型F-11细胞系行为进行可扩展热刺激的新方法所获得的结果。最初，我们在培养箱中针对不同时间间隔和温度进行了批量刺激实验，在41.5°C下暴露30分钟的培养物中观察到神经突伸长和电生理特性存在显著差异。因此，我们使用近红外激光照射粘附在培养皿外表面的普鲁士蓝纳米颗粒和聚乙烯醇圆盘，使培养物经历相同的温度升高。在受照射的细胞中，与对照组相比，神经突明显更长，电生理特性（动作电位发放频率和自发活动）显著增强。这些结果首次表明，靶向热刺激可诱导神经元的形态和功能分化，并支持该方法未来作为一种在体内改变神经元行为的策略加以应用。
