Key Laboratory of Ecological Restoration of Regional Pollution Environment, Ministry of Education, Shenyang University, Shenyang, 110004, China.
Key Laboratory of Ecological Restoration of Regional Pollution Environment, Ministry of Education, Shenyang University, Shenyang, 110004, China.
Chemosphere. 2022 Nov;307(Pt 1):135609. doi: 10.1016/j.chemosphere.2022.135609. Epub 2022 Jul 7.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous persistent organic pollutants in soil, which have carcinogenic, teratogenic and mutagenic hazards. The effects of rhamnolipid (RL), nano zero-valent iron (nZVI), and anthraquinone-2,6-disulfonic acid (AQDS) on the degradation of PAHs in soil were studied. It was found that the treatment of 5 mg·kgRL + 1% nZVI +0.2 mmol·kgAQDS had the highest degradation rate. The degradation rate of total PAHs and HMW-PAHs was 72.81% and 79.47% respectively after 90 days. High-throughput sequencing showed that in RL + nZVI + AQDS enhanced soil, Clostridium, Geobacter, Anaeromyxobacter and Sphingomonas were the dominant species for anaerobic degradation of PAHs. Rhodococcus, Nocardioides, and Microvirga are the dominant species for aerobic degradation of PAHs. The activities of methyltransferase, dehydrogenase and catechol 1,2-dioxygenase in the anaerobic-aerobic degradation process of PAHs were consistent with the degradation process of PAHs, indicating the role of these enzymes in the degradation of PAHs. RL, nZVI, and AQDS combined enhanced microbial anaerobic-aerobic degradation has great application potential in remediation of PAHs-contaminated soil.
多环芳烃（PAHs）是土壤中普遍存在的持久性有机污染物，具有致癌、致畸和致突变的危害。本研究考察了鼠李糖脂（RL）、纳米零价铁（nZVI）和蒽醌-2,6-二磺酸（AQDS）对土壤中 PAHs 降解的影响。结果表明，5mg·kgRL+1%nZVI+0.2mmol·kgAQDS 处理的降解率最高。90 天后，总 PAHs 和 HMW-PAHs 的降解率分别达到 72.81%和 79.47%。高通量测序结果表明，在 RL+nZVI+AQDS 强化土壤中，Clostridium、Geobacter、Anaeromyxobacter 和 Sphingomonas 是 PAHs 厌氧降解的优势种。Rhodococcus、Nocardioides 和 Microvirga 是 PAHs 好氧降解的优势种。PAHs 厌氧-好氧降解过程中甲基转移酶、脱氢酶和儿茶酚 1,2-双加氧酶的活性与 PAHs 的降解过程一致，表明这些酶在 PAHs 的降解中起作用。RL、nZVI 和 AQDS 联合强化微生物厌氧-好氧降解在修复 PAHs 污染土壤方面具有巨大的应用潜力。
