Tetramethylpyrazine supplementation improves performance, digestion, blood and immune state of broilers exposure to oxidative stress.

This study aimed to investigate the effect of dietary tetramethylpyrazine (TMP) on the growth performance, nutrient digestion, blood parameters and immunity of broilers under oxidative stress. Five treatments included negative control, positive control with lipopolysaccharide induction and TMP addition at 50, 100 and 150 mg/kg of diet using 600 male Arbor Acres broiler chicks. Results showed that during 1-14 days of age, body weight gain and feed efficiency in the positive control were worsened (p < .05) compared with the negative control, while with incremental TMP doses from 0 to 150 mg/kg there were linear and quadratic increases (p < .001) in body weight gain and a linear decrease in feed/gain (p = .001). During 12-14 days of age, with incremental TMP doses, crude protein digestibility was linearly increased (p = .001), and gross energy utilization was linearly and quadratically changed (p < .001). At 14 days of age, the TMP beneficially regulated digestive enzymes, blood parameters and immunoglobulins, showing linear and quadratic responses (p ≤ .008) on trypsin, lipase, glutamic pyruvic transaminase, glucose, lipoproteins, albumin, immunoglobulins (M, Y), interleukin 6 and interferon α, and only linear changes (p ≤ .030) on amylase, glutamic oxaloacetic transaminase, immunoglobulin A and interleukin 2. Most parameters in TMP groups reached to the levels of negative control and the effects of TMP at 100 or 150 mg/kg were more pronounced on body weight gain, crude protein digestibility, trypsin and glutamic pyruvic transaminase. It is concluded that TMP can be used as a feed additive capable of improving growth, blood parameter and immunity of broiler chicks under oxidative stress.