Hu Hong, Bai Xi, Xu Kexing, Zhang Cheng, Chen Liang
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China.
College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China.
Poult Sci. 2021 Aug;100(8):101217. doi: 10.1016/j.psj.2021.101217. Epub 2021 Apr 24.
The objective of this work was to evaluate the effect of phloretin on growth performance, serum biochemical parameters, antioxidant profile, glutathione (GSH)-related enzymes, nuclear factor erythroid 2-related 2 (Nrf2) and heat shock protein 70 (HSP70) in heat-stressed broilers. A total of 240, 22-day-old Arbor Acres broilers were divided into 4 groups. The control group was housed at 23.0 ± 0.61°C and fed with basal diet, while the 3 heat-stressed groups (A, B, and C groups) were housed at 30.5 ± 0.69°C and fed with basal diet containing 0, 100, and 200 mg/kg phloretin, respectively. Serum was taken form 42-day-old broilers. Results showed that heat stress decreased (P < 0.05) the final body weight (FBW), body weight gain (BWG), feed intake (FI), serum total protein (TP), triglyceride (TG), triiodothyronine (T3), thyroxine (T4), GSH, catalase (CAT), and total antioxidant capacity (T-AOC) levels, but increased (P < 0.05) the feed-to-gain ratio (FGR) and serum malondialdehyde (MDA) levels in broilers compared with that in the control group. Among the heat-stressed groups, supplementary 200 mg/kg phloretin increased (P < 0.05) the FBW, BWG, FI, serum TP, TG, T4, GSH, CAT, and T-AOC levels, and decreased (P < 0.05) the FGR and serum MDA in broilers. There were significant decreases (P < 0.05) in the glutathione peroxidase (GSH-Px), γ-glutamylcysteine synthetase (γ-GCS), and Nrf2, but significant increases (P < 0.05) in the HSP70 of the broiler serum after heat stress treatment. Among the heat-stressed groups, supplementary 200 mg/kg phloretin increased (P < 0.05) the GSH-Px, γ-GCS, and Nrf2 levels, but decreased (P < 0.05) the serum HSP70 level in the heat-stressed broilers. Under high temperature condition, FBW, BWG, FI, FGR, serum TP, TG, T4, MDA, GSH, CAT, T-AOC, GSH-Px, γ-GCS, Nrf2 and HSP70 were linearly affected by inclusion of phloretin. These results indicated that phloretin may improve growth performance, serum parameters, and antioxidant profiles through regulated GSH-related enzymes, Nrf2 and HSP70 in heat-stressed broilers.
本研究旨在评估根皮素对热应激肉鸡生长性能、血清生化参数、抗氧化指标、谷胱甘肽（GSH）相关酶、核因子E2相关因子2（Nrf2）和热休克蛋白70（HSP70）的影响。选取240只22日龄的艾维茵肉鸡，分为4组。对照组饲养于23.0±0.61°C，饲喂基础日粮；3个热应激组（A、B和C组）饲养于30.5±0.69°C，分别饲喂含0、100和200mg/kg根皮素的基础日粮。采集42日龄肉鸡的血清。结果显示，与对照组相比，热应激使肉鸡的末体重（FBW）、体重增重（BWG）、采食量（FI）、血清总蛋白（TP）、甘油三酯（TG）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、GSH、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）水平降低（P<0.05），但料重比（FGR）和血清丙二醛（MDA）水平升高（P<0.05）。在热应激组中，添加200mg/kg根皮素可使肉鸡的FBW、BWG、FI、血清TP、TG、T4、GSH、CAT和T-AOC水平升高（P<0.05），FGR和血清MDA水平降低（P<0.05）。热应激处理后，肉鸡血清中的谷胱甘肽过氧化物酶（GSH-Px）、γ-谷氨酰半胱氨酸合成酶（γ-GCS）和Nrf2显著降低（P<0.05），但HSP70显著升高（P<0.05）。在热应激组中，添加200mg/kg根皮素可使热应激肉鸡的GSH-Px、γ-GCS和Nrf2水平升高（P<0.05），血清HSP70水平降低（P<0.05）。在高温条件下，添加根皮素对FBW、BWG、FI、FGR、血清TP、TG、T4、MDA、GSH、CAT、T-AOC、GSH-Px、γ-GCS、Nrf2和HSP70呈线性影响。这些结果表明，根皮素可能通过调节热应激肉鸡中与GSH相关的酶、Nrf2和HSP70来改善生长性能、血清参数和抗氧化指标。
