Kang Yulin, Zhai Xiaohui, Lu Sifen, Vuletic Ivan, Wang Lin, Zhou Kun, Peng Zhiqiang, Ren Qiushi, Xie Zhaoheng
Institute of Environmental Information, Chinese Research Academy of Environmental Sciences, Beijing, China.
Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.
Front Oncol. 2022 Jun 22;12:772392. doi: 10.3389/fonc.2022.772392. eCollection 2022.
Multimodality imaging is an advanced imaging tool for monitoring tumor behavior and therapy . In this study, we have developed a novel hybrid tri-modality system that includes two molecular imaging methods: positron emission computed tomography (PET) and fluorescence molecular imaging (FMI) and the anatomic imaging modality X-ray computed tomography (CT). The following paper describes the system development. Also, its imaging performance was tested (phantom) and , in Balb/c nude mice bearing a head and neck tumor xenograft treated with novel gene therapy [a new approach to the delivery of recombinant bacterial gene (IL-24-expressing strain)]. Using the tri-modality imaging system, we simultaneously monitored the therapeutic effect, including the apoptotic and necrotic induction within the tumor . The apoptotic induction was examined in real-time using an F-ML-10 tracer; the cell death was detected using ICG. A CT was used to evaluate the anatomical situation. An increased tumor inhibition (including tumor growth and tumor cell apoptosis) was observed in the treatment group compared to the control groups, which further confirmed the therapeutic effect of a new IL-24-expressing strain gene therapy on the tumor . By being able to offer concurrent morphological and functional information, our system is able to characterize malignant tissues more accurately. Therefore, this new tri-modality system (PET/CT/FMI) is an effective imaging tool for simultaneously investigating and monitoring tumor progression and therapy outcomes .
多模态成像是一种用于监测肿瘤行为和治疗的先进成像工具。在本研究中，我们开发了一种新型的混合三模态系统，该系统包括两种分子成像方法：正电子发射计算机断层扫描（PET）和荧光分子成像（FMI）以及解剖成像模态X射线计算机断层扫描（CT）。以下论文描述了该系统的开发过程。此外，还对其成像性能进行了测试（体模），并在携带头颈部肿瘤异种移植的Balb/c裸鼠中进行了测试，这些裸鼠接受了新型基因治疗[一种递送重组细菌基因（表达IL-24的菌株）的新方法]。使用三模态成像系统，我们同时监测了治疗效果，包括肿瘤内的凋亡和坏死诱导情况。使用F-ML-10示踪剂实时检测凋亡诱导情况；使用吲哚菁绿（ICG）检测细胞死亡情况。使用CT评估解剖学状况。与对照组相比，治疗组观察到肿瘤抑制作用增强（包括肿瘤生长和肿瘤细胞凋亡），这进一步证实了新的表达IL-24的菌株基因治疗对肿瘤的治疗效果。通过能够提供同时的形态学和功能信息，我们的系统能够更准确地表征恶性组织。因此，这种新的三模态系统（PET/CT/FMI）是一种用于同时研究和监测肿瘤进展及治疗结果的有效成像工具。
