Technische Universität München and Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Neuherberg, Germany.
Nat Methods. 2012 Jun;9(6):615-20. doi: 10.1038/nmeth.2014. Epub 2012 May 6.
The development of hybrid optical tomography methods to improve imaging performance has been suggested over a decade ago and has been experimentally demonstrated in animals and humans. Here we examined in vivo performance of a camera-based hybrid fluorescence molecular tomography (FMT) system for 360° imaging combined with X-ray computed tomography (XCT). Offering an accurately co-registered, information-rich hybrid data set, FMT-XCT has new imaging possibilities compared to stand-alone FMT and XCT. We applied FMT-XCT to a subcutaneous 4T1 tumor mouse model, an Aga2 osteogenesis imperfecta model and a Kras lung cancer mouse model, using XCT information during FMT inversion. We validated in vivo imaging results against post-mortem planar fluorescence images of cryoslices and histology data. Besides offering concurrent anatomical and functional information, FMT-XCT resulted in the most accurate FMT performance to date. These findings indicate that addition of FMT optics into the XCT gantry may be a potent upgrade for small-animal XCT systems.
十多年前就有人提出开发混合光学层析成像方法来提高成像性能，并在动物和人体中进行了实验验证。在这里，我们检查了基于相机的混合荧光分子层析成像（FMT）系统的体内性能，该系统可进行 360°成像，并结合 X 射线计算机断层扫描（XCT）。与独立的 FMT 和 XCT 相比，FMT-XCT 提供了准确配准、信息丰富的混合数据集，具有新的成像可能性。我们将 FMT-XCT 应用于皮下 4T1 肿瘤小鼠模型、Aga2 成骨不全症模型和 Kras 肺癌小鼠模型，在 FMT 反演过程中使用 XCT 信息。我们将体内成像结果与冷冻切片的死后平面荧光图像和组织学数据进行了验证。除了提供同时的解剖学和功能信息外，FMT-XCT 还实现了迄今为止最准确的 FMT 性能。这些发现表明，将 FMT 光学元件添加到 XCT 龙门架中可能是小型动物 XCT 系统的强大升级。
