Sawicki Rafał, Widelski Jarosław, Okińczyc Piotr, Truszkiewicz Wiesław, Glous Joanna, Sieniawska Elwira
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, Poland.
Department of Pharmacognosy, Medical University of Lublin, Lublin, Poland.
Front Microbiol. 2022 Jun 23;13:929476. doi: 10.3389/fmicb.2022.929476. eCollection 2022.
Propolis is a natural product proved to be efficient against . Although it is produced by bees, its active alcoholic-aqueous fraction contains plant-derived molecules. To gain some insight into its mechanism of antimycobacterial activity, we studied the metabolic changes in bacterial cells treated with extract of sp. propolis from Nepal. The detailed metabolomic and transcriptomic analysis performed in this study indicated target points in bacterial cells under propolis extract influence. The profile of lipids forming the outer and middle layer of the mycobacterial cell envelope was not changed by propolis treatment, however, fluctuations in the profiles of amphipathic glycerophospholipids were observed. The enrichment analysis revealed bacterial metabolic pathways affected by sp. propolis treatment. The early metabolic response involved much more pathways than observed after 48 h of incubation, however, the highest enrichment ratio was observed after 48 h, indicating the long-lasting influence of propolis. The early bacterial response was related to the increased demand for energy and upregulation of molecules involved in the formation of the cell membrane. The transcriptomic analysis confirmed that bacteria also suffered from oxidative stress, which was more pronounced on the second day of exposure. This was the first attempt to explain the action of Nepalese propolis extract against mycobacteria.
蜂胶是一种经证实对……有效的天然产物。尽管它是由蜜蜂生产的，但其活性醇 - 水部分含有植物来源的分子。为了深入了解其抗分枝杆菌活性机制，我们研究了用尼泊尔……蜂胶提取物处理的细菌细胞中的代谢变化。本研究中进行的详细代谢组学和转录组学分析表明了在蜂胶提取物影响下细菌细胞中的靶点。蜂胶处理并未改变构成分枝杆菌细胞膜外层和中层的脂质谱，但观察到两亲性甘油磷脂谱的波动。富集分析揭示了受……蜂胶处理影响的细菌代谢途径。早期代谢反应涉及的途径比孵育48小时后观察到的要多得多，然而，在48小时后观察到最高的富集率，表明蜂胶具有持久影响。细菌的早期反应与能量需求增加以及参与细胞膜形成的分子上调有关。转录组学分析证实细菌也遭受氧化应激，在暴露的第二天更为明显。这是首次尝试解释尼泊尔蜂胶提取物对分枝杆菌作用的研究。
