Scheller S, Dworniczak S, Waldemar-Klimmek K, Rajca M, Tomczyk A, Shani J
Department of Microbiology and Immunology, Silesian Academy of Medicine, Zabrze-Rokitnica, Poland.
Z Naturforsch C J Biosci. 1999 Jul-Aug;54(7-8):549-53. doi: 10.1515/znc-1999-7-814.
Ethanolic extract of propolis exerts a strong anti-bacterial activity, in addition to antifungal, antiviral and antiprotozoal properties. In previous studies from these laboratories we have demonstrated that the intensity of the bactericidal activity of EEP is correlated with the virulence of the mycobacteria tested, and that EEP has a synergistic effect with antibiotics on growth of staphylococcus aureus. In the present study we investigated whether the same synergism and correlation exists between EEP and some anti-tuberculosis drugs on tuberculosis mycobacteria with different degrees of virulence. Six standard strains and 11 wild strains of mycobacteria were exposed for 30 days to EEP, with or without streptomycin, rifamycin, isoniazid or ethambutol. Out of the 17 strains, 8 were resistant to at least two standard antibiotics, and were considered "multi-resistant strains". The rest were either susceptible or resistant to only one of the antimycobacterial drugs. Antagonism was recorded only in one case, when Staphylococcus aureus were treated with a mixture of EEP and ethambutol, suggesting that a chemical bond could have been formed between this anti-tuberculosis antibiotic and one of the active components of the ethanol extract of propolis.
蜂胶乙醇提取物除具有抗真菌、抗病毒和抗原虫特性外，还具有很强的抗菌活性。在这些实验室之前的研究中，我们已经证明EEP的杀菌活性强度与所测试分枝杆菌的毒力相关，并且EEP与抗生素对金黄色葡萄球菌的生长具有协同作用。在本研究中，我们调查了EEP与一些抗结核药物对不同毒力的结核分枝杆菌之间是否存在相同的协同作用和相关性。将6株标准菌株和11株分枝杆菌野生菌株暴露于EEP 30天，同时添加或不添加链霉素、利福霉素、异烟肼或乙胺丁醇。在这17株菌株中，8株对至少两种标准抗生素耐药，被认为是“多重耐药菌株”。其余菌株对仅一种抗分枝杆菌药物敏感或耐药。仅在一例中记录到拮抗作用，即当用EEP和乙胺丁醇的混合物处理金黄色葡萄球菌时，这表明这种抗结核抗生素与蜂胶乙醇提取物的一种活性成分之间可能形成了化学键。
