Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Plant Dis. 2023 Feb;107(2):450-456. doi: 10.1094/PDIS-07-22-1545-RE. Epub 2023 Feb 7.
Powdery mildew, caused by f. sp. (), poses a severe threat to wheat yield and quality worldwide. Rapid identification and the accurate transference of effective resistance genes are important to the development of resistant cultivars and the sustainable control of this disease. In the present study, the wheat line AL11 exhibited high levels of resistance to powdery mildew at both the seedling and adult plant stages. Genetic analysis of the AL11 × 'Shixin 733' mapping population revealed that its resistance was controlled by a single dominant gene, tentatively designated . Using bulked segregant RNA-Seq and molecular marker analysis, was mapped to the locus on chromosome 7B where it cosegregated with the functional marker . Sequence alignment analysis revealed that the -homologous sequence in AL11 was identical to the reported recessive gene in wheat landrace 'Fuzhuang 30'. It appears that was most probably , but it was mediated by a dominant inheritance pattern, so it should provide a valuable resistance resource for both genetic study and wheat breeding. To efficiently use and trace in breeding, a new kompetitive allele-specific PCR marker that cosegregated with this gene was developed and confirmed to be applicable in the different wheat backgrounds, thus promoting its use in the marker-assisted selection of .
小麦白粉病由 f. sp. ()引起，是全球范围内威胁小麦产量和品质的严重病害。快速鉴定和有效转移抗性基因对于培育抗性品种和可持续控制该病害至关重要。本研究中，小麦品系 AL11 在幼苗期和成株期均表现出对白粉病的高抗性。AL11בShixin 733’定位群体的遗传分析表明，其抗性由一个显性单基因控制，暂命名为 。利用混池分离群体 RNA-Seq 和分子标记分析，将 定位到 7B 染色体上的 位点，该位点与功能标记 共分离。序列比对分析表明，AL11 中的 -同源序列与小麦地方品种‘Fuzhuang 30’中报道的隐性基因 完全一致。因此， 很可能是 ，但它是通过显性遗传模式介导的，因此它应该为遗传研究和小麦育种提供有价值的抗性资源。为了在育种中有效利用和追踪 ，开发了与该基因共分离的新的竞争等位基因特异性 PCR 标记 ，并证实该标记在不同小麦背景下均适用，从而促进了其在 的标记辅助选择中的应用。
