State Key Laboratory of Crop Stress Adaptation and Improvement, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng, Henan 475004, China.
College of Life Sciences, Yantai University, Yantai, Shandong 264005, China.
Plant Dis. 2021 Oct;105(10):3127-3133. doi: 10.1094/PDIS-12-20-2719-RE. Epub 2021 Oct 29.
Wheat powdery mildew, caused by f. sp. , is a devastating disease that threatens yield and quality. Host resistance is considered the most effective and preferred means to control this disease. Wheat landrace Duanganmang (DGM) showed high resistance or near immunity to f. sp. mixture from Henan Province, China. DGM was crossed with highly susceptible Chinese wheat landrace Huixianhong (HXH) and cultivar 'Shimai 15' (SM15) to produce genetic populations. The resistance of DGM to f. sp. isolate E09 was shown to be controlled by a single dominant Mendelian factor, tentatively designated . Marker analysis and 55K single nucleotide polymorphism (SNP) array scanning showed that this gene was positioned in the interval (2.4 cM or 1.61 Mb) flanked by and in the Chinese Spring reference genome. Homology-based cloning and sequence analysis demonstrated that DGM has the identical gene () and gene reported in Fuzhuang 30 (FZ30), conferring and modifying powdery mildew resistance, respectively. However, based on the different reaction patterns to the f. sp. isolate B15 between DGM and FZ30, the authors speculate that DGM may have two tightly linked genes that could not be separated in the current mapping population, one of which is and the other being . Hence, this study provides a valuable resistance resource for improvement of powdery mildew resistance.
小麦白粉病由 f. sp. 引起，是一种严重威胁产量和品质的病害。利用宿主抗性来控制该病害被认为是最有效和首选的方法。小麦地方品种段湾芒（DGM）对来自中国河南省的 f. sp. 混合物表现出高抗性或近免疫性。DGM 与高度感病的中国小麦地方品种洧川红（HXH）和品种‘师麦 15’（SM15）杂交，产生遗传群体。DGM 对 f. sp. 分离物 E09 的抗性由单个显性孟德尔因子控制，暂定名为 。标记分析和 55K 单核苷酸多态性（SNP）阵列扫描表明，该基因位于中国春参考基因组中 和 之间的 区间（2.4 cM 或 1.61 Mb）侧翼。基于同源克隆和序列分析表明，DGM 具有与 Fuzhuang 30（FZ30）相同的 基因（）和 基因，分别赋予和修饰白粉病抗性。然而，根据 DGM 和 FZ30 对 f. sp. 分离物 B15 的不同反应模式，作者推测 DGM 可能有两个紧密连锁的基因，在当前的作图群体中无法分离，其中一个是 ，另一个是 。因此，本研究为提高白粉病抗性提供了有价值的抗性资源。
