Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005479.
During an investigation of rare actinobacteria, isolate SC076 was isolated from a soil sample collected from Sichang Island, Chonburi Province, Thailand. The strain showed the highest 16S rRNA gene similarity to DSM 43800 (98.6%) and DSM 44229 (98.6%). The zigzag morphology of the spore chain was observed on the aerial mycelia. Diaminopimelic acid was detected in the peptidoglycan. Whole-cell sugars contained rhamnose, ribose, mannose glucose and galactose. Polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, unidentified ninhydrin-positive glycolipid, unidentified glycolipid and four unidentified lipids. The menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The predominant fatty acids were iso-C, iso-C and anteiso-C. The draft genome of SC076 was 8040245 bp with a G+C content of 72.5 mol%. The results of genomic analysis between strain SC076 and the related type strains showed that the digital DNA-DNA hybridization and average nucleotide identity values among the strains were 23.6-32.8% and 77.7-86.8 %, respectively, which are lower than the thresholds used to distinguish strains from others of the same species. Based on the taxonomic evidence, strain SC076 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is SC076 (=TBRC 14540=NBRC 115117).
在对稀有放线菌的研究中，从泰国春武里府锡昌岛的土壤样本中分离到了一株菌株 SC076。该菌株与 DSM 43800（98.6%）和 DSM 44229（98.6%）的 16S rRNA 基因相似度最高。在气生菌丝上观察到孢子链的之字形形态。在肽聚糖中检测到二氨基庚二酸。全细胞糖含有鼠李糖、核糖、甘露糖、葡萄糖和半乳糖。极性脂含有磷脂酰乙醇胺、羟基磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰肌醇、未鉴定的水合茚三酮阳性糖脂、未鉴定的糖脂和四种未鉴定的脂类。menaquinones 为 MK-9(H)、MK-9(H)、MK-9(H)和 MK-9(H)。主要脂肪酸为 iso-C、iso-C 和 anteiso-C。SC076 的草图基因组为 8040245 bp，G+C 含量为 72.5 mol%。菌株 SC076 与相关模式菌株的基因组分析结果表明，菌株间的数字 DNA-DNA 杂交值和平均核苷酸同一性值分别为 23.6-32.8%和 77.7-86.8%，均低于用于区分同种菌株的阈值。基于分类学证据，菌株 SC076 代表一个新的属种，提议命名为 sp. nov.。模式菌株为 SC076（=TBRC 14540=NBRC 115117）。
