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由多重耐药质粒编码的低分子量RNA种类。

Low molecular weight RNA species encoded by a multiple drug resistance plasmid.

作者信息

De Wilde M, Davies J E, Schmidt F J

出版信息

Proc Natl Acad Sci U S A. 1978 Aug;75(8):3673-7. doi: 10.1073/pnas.75.8.3673.

DOI:10.1073/pnas.75.8.3673
PMID:358192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392848/
Abstract

Multiple drug resistance plasmid NR1 is shown to code for at least 10 low molecular weight RNAs. These species, ranging in size from 60 to 120 nucleotides, have been purified from minicells by two-dimensional gel electrophoresis and characterized by RNase T1 fingerprinting. Hybridization of purified RNAs to restriction endonuclease digests of NR1 DNA indicates that most are derived from the resistance transfer factor region of the plasmid genome. One RNA was found to be coded by the transposable tetracycline resistance element Tn10, and several are associated with DNA fragments that contain origins of replication.

摘要

多重耐药质粒NR1被证明可编码至少10种低分子量RNA。这些RNA分子大小在60至120个核苷酸之间，已通过二维凝胶电泳从小细胞中纯化出来，并通过核糖核酸酶T1指纹图谱进行了表征。纯化后的RNA与NR1 DNA的限制性内切酶消化产物杂交表明，大多数RNA源自质粒基因组的抗性转移因子区域。发现一种RNA由可转座的四环素抗性元件Tn10编码，还有几种与包含复制起点的DNA片段相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/3cfc085be794/pnas00020-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/3990bf2ad6c2/pnas00020-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/d1cb33598e3d/pnas00020-0144-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/0fb1fa0b7e01/pnas00020-0144-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/62dec25ebfd0/pnas00020-0144-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/65a96b416a78/pnas00020-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/75d8b97d675b/pnas00020-0145-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/e958b0d75271/pnas00020-0145-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/f48ef48b7144/pnas00020-0145-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/a9d37f60d771/pnas00020-0145-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/7b1a9b75b3c5/pnas00020-0145-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/3cfc085be794/pnas00020-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/3990bf2ad6c2/pnas00020-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/d1cb33598e3d/pnas00020-0144-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/0fb1fa0b7e01/pnas00020-0144-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/62dec25ebfd0/pnas00020-0144-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/65a96b416a78/pnas00020-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/75d8b97d675b/pnas00020-0145-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/e958b0d75271/pnas00020-0145-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/f48ef48b7144/pnas00020-0145-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/a9d37f60d771/pnas00020-0145-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/7b1a9b75b3c5/pnas00020-0145-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f66/392848/3cfc085be794/pnas00020-0146-a.jpg

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Proc Natl Acad Sci U S A. 1978 Aug;75(8):3673-7. doi: 10.1073/pnas.75.8.3673.
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本文引用的文献

1
Resistance transfer agents in Shigella.志贺氏菌中的抗性质粒转移因子
Biochem Biophys Res Commun. 1960 Dec;3:654-9. doi: 10.1016/0006-291x(60)90081-4.
2
The participation of sRNA in the enzymatic synthesis of O-L-lysyl phosphatidylgylcerol in Staphylococcus aureus.小RNA在金黄色葡萄球菌中O-L-赖氨酰磷脂酰甘油酶促合成中的作用
Proc Natl Acad Sci U S A. 1966 Apr;55(4):934-41. doi: 10.1073/pnas.55.4.934.
3
Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12.大肠杆菌K - 12含质粒微小细胞中核糖核酸和蛋白质的合成。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6008-12. doi: 10.1073/pnas.78.10.6008.
4
Transcription of bacteriocinogenic plasmid CloDF13 in vivo and in vitro: structure of the cloacin immunity operon.产细菌素质粒CloDF13在体内和体外的转录：杀梭菌素免疫操纵子的结构
J Bacteriol. 1980 Nov;144(2):579-91. doi: 10.1128/jb.144.2.579-591.1980.
5
Identification and classification of bacterial plasmids.细菌质粒的鉴定与分类
Microbiol Rev. 1988 Sep;52(3):375-95. doi: 10.1128/mr.52.3.375-395.1988.
6
Transcriptional pausing in a region important for plasmid NR1 replication control.在对质粒NR1复制控制至关重要的区域中的转录暂停。
J Bacteriol. 1987 Dec;169(12):5353-63. doi: 10.1128/jb.169.12.5353-5363.1987.
7
Characterization of the in vivo RNA product of the pOUT promoter of IS10R.IS10R的pOUT启动子的体内RNA产物的表征
J Bacteriol. 1985 Nov;164(2):556-62. doi: 10.1128/jb.164.2.556-562.1985.
8
Control of replication of plasmid R1: the duplex between the antisense RNA, CopA, and its target, CopT, is processed specifically in vivo and in vitro by RNase III.质粒R1复制的控制：反义RNA CopA与其靶标CopT之间的双链体在体内和体外均由RNase III特异性加工。
EMBO J. 1990 Jul;9(7):2331-40. doi: 10.1002/j.1460-2075.1990.tb07405.x.
J Bacteriol. 1971 Jul;107(1):21-33. doi: 10.1128/jb.107.1.21-33.1971.
4
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J Mol Biol. 1967 Nov 14;29(3):457-71. doi: 10.1016/0022-2836(67)90112-x.
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Conditionally lethal mutants of bacteriophage T4 defective in production of a transfer RNA.在转移RNA产生方面存在缺陷的噬菌体T4条件致死突变体。
J Mol Biol. 1973 Dec 5;81(2):137-55. doi: 10.1016/0022-2836(73)90185-x.
6
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.大肠杆菌质粒间序列关系的电子显微镜异源双链研究。II. 耐药性（R）因子和F因子的结构
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J Biol Chem. 1973 Jul 25;248(14):5024-32.
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