从野生持久体中获取和维持松材线虫（Bursaphelenchus xylophilus）培养物
Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers.
作者信息
Pimentel Carla S, Firmino Paulo N, Ayres Matthew P
机构信息
Forest Research Centre (CEF), School of Agriculture, University of Lisbon, Lisbon, Portugal.
Department of Biological Sciences, Dartmouth College, Hanover, NH, USA.
出版信息
Methods Mol Biol. 2022;2536:3-11. doi: 10.1007/978-1-0716-2517-0_1.
The establishment of laboratory isolates of the pinewood nematode Bursaphelenchus xylophilus, the causal agent of the pine wilt disease, has been crucial to research on this important forest pathogen. Here we describe a simple, low-cost, and easy way to obtain samples of wild populations of B. xylophilus by culturing dauers extracted directly from the insect vector.
松材线虫（Bursaphelenchus xylophilus）是松材线虫病的致病因子，其实验室分离株的建立对于研究这种重要的森林病原体至关重要。在此，我们描述了一种简单、低成本且易于操作的方法，即通过培养直接从昆虫媒介中提取的滞育幼虫来获取野生松材线虫种群的样本。
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