Tegli Stefania, Gaudioso Dario, Stefanucci Damiano
Department of Agriculture, Food, Environment and Forestry (DAGRI), Molecular Plant Pathology Lab, University of Florence, Florence, Italy.
Methods Mol Biol. 2022;2536:251-261. doi: 10.1007/978-1-0716-2517-0_15.
Specific, sensitive, and rapid detection of quarantine and regulated plant pathogens is pivotal for the control of the diseases they cause. Here, we describe the PCR-based methods which have been developed for Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), quarantine plant pathogenic bacterium for EU and causal agent of bacterial wilt of Leguminous plants. These methods include an end-point and a real-time PCR test, and a LAMP assay. Their threshold analytical limits range from 100 to 10 fg of DNA template per reaction and are currently used worldwide for routine testing for Cff from laboratory to field scale.
特异性、灵敏且快速地检测检疫性和受管制的植物病原体对于控制它们所引发的疾病至关重要。在此，我们描述了基于PCR的方法，这些方法是针对萎蔫短小杆菌萎蔫致病变种（Cff）开发的，Cff是欧盟的检疫性植物致病细菌，也是豆科植物细菌性萎蔫病的病原体。这些方法包括终点PCR和实时PCR检测以及环介导等温扩增（LAMP）检测。它们每个反应的DNA模板阈值分析限为100至10 fg，目前在全球范围内用于从实验室到田间规模的Cff常规检测。
