School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China.
Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz 71441-65186, Iran.
Phytopathology. 2021 Feb;111(2):268-280. doi: 10.1094/PHYTO-06-20-0243-R. Epub 2020 Dec 1.
Bacterial wilt of dry beans (family Fabaceae) caused by the actinobacterial agent pv. is one of the most important diseases threatening edible legume production around the globe. Despite the economic losses due to the bacterial wilt disease, the pathogen has not so far been investigated for its genomic features, pathogenicity determinants, and virulence strategies. Here we present the first complete genome sequence of a highly virulent bacteriocin-producing pv. strain P990. The bacterium has a circular chromosome consisting of 3,736 kbp with the G+C% content of 71.0%. Furthermore, a 147-kbp circular plasmid (pCff1) with 66.1% G+C% content as well as two circular plasmid-like DNAs with sizes of 25 and 22 kbp were detected within the genomic contents of pv. . Phylogenetic analyses revealed that only a few number of sp. strains deposited in the public databases could be classified within the species . Comparative genomics of pv. using the genome sequences of actinobacterial plant pathogens revealed the presence of a set of unique low G+C% content genomic islands in the pv. genome. Homologs of pathogenicity-determinant loci capable of producing 1,4-beta-xylanase (), pectate lyase ( and ), serine protease (, , and ), and sortase () were detected in pv. genome. The genomic data presented here extend our understanding of the pv. genomic features and pave the ways of research on functional and interaction genetics to combat the risk of bacterial wilt disease in the 21st century's dry bean industry.
菜豆细菌性萎蔫病（豆科）由放线菌病原体 pv. 引起，是全球威胁食用豆科植物生产的最重要疾病之一。尽管细菌性萎蔫病造成了经济损失，但迄今为止，该病原体的基因组特征、致病性决定因素和毒力策略尚未得到研究。在这里，我们呈现了一种高毒力细菌素产生 pv. P990 菌株的首个完整基因组序列。该细菌有一个由 3736 kbp 组成的圆形染色体，G+C%含量为 71.0%。此外，在 pv. 基因组内容中还检测到一个 147-kbp 的圆形质粒（pCff1），其 G+C%含量为 66.1%，以及两个大小为 25 和 22 kbp 的圆形质粒样 DNA。系统发育分析表明，只有少数在公共数据库中保存的 sp. 菌株能够被分类到. 种内。利用放线菌植物病原体的基因组序列对 pv. 进行比较基因组学分析，发现 pv. 基因组中存在一组独特的低 G+C%含量基因组岛。在 pv. 基因组中检测到了致病决定因子基因座的同源物，这些基因座能够产生 1,4-beta-木聚糖酶（）、果胶裂解酶（和）、丝氨酸蛋白酶（、、和）和天冬酰胺酶（）。这里呈现的基因组数据扩展了我们对 pv. 基因组特征的理解，并为功能和相互作用遗传学的研究铺平了道路，以应对 21 世纪干豆产业中细菌性萎蔫病的风险。
