University of Eastern Finland, Department of Environmental and Biological Sciences, Kuopio, Finland.
University of Eastern Finland, Department of Environmental and Biological Sciences, Kuopio, Finland; Finnish Institute for Health and Welfare, Environmental Health Unit, Kuopio, Finland.
Bioelectrochemistry. 2022 Oct;147:108196. doi: 10.1016/j.bioelechem.2022.108196. Epub 2022 Jul 2.
The objective of this study was to evaluate whether static or 50 Hz magnetic fields (MFs) modify responses to the chemotherapeutic agent doxorubicin in human MCF-7 breast cancer cells. To this end, cells were exposed to static or 50 Hz MFs at 100 µT with or without doxorubicin for 3 h. Following the exposures, cytosolic and mitochondrial superoxide levels, DNA damage levels, and the clonogenic survival of the cells were evaluated. It was found that static MFs decreased the DNA damage level induced by doxorubicin treatment (p = 0.023), but no effects were observed for either cytosolic and mitochondrial superoxide levels or the clonogenic survival of the cells. On the other hand, 50 Hz MF increased doxorubicin-induced cytosolic superoxide levels (p = 0.016), while the mitochondrial superoxide level, DNA damage level, and clonogenic survival were unaffected. In conclusion, we found that static and 50 Hz MFs may modify responses to doxorubicin treatment, but the subsequent survival of the doxorubicin-treated cancer cells was unaffected by both types of MFs. Therefore, the present results suggest that static or 50 Hz MFs for 3 h do not modify the efficacy of doxorubicin in MCF-7 cancer cells.
本研究旨在评估静态或 50Hz 磁场（MFs）是否会改变人 MCF-7 乳腺癌细胞对阿霉素化疗药物的反应。为此，将细胞暴露于 100µT 的静态或 50Hz MFs 中，同时或不与阿霉素一起暴露 3 小时。暴露后，评估细胞的胞质和线粒体超氧化物水平、DNA 损伤水平和克隆形成存活能力。结果发现，静态 MFs 降低了阿霉素处理引起的 DNA 损伤水平（p=0.023），但对胞质和线粒体超氧化物水平或细胞的克隆形成存活能力没有影响。另一方面，50Hz MF 增加了阿霉素诱导的胞质超氧化物水平（p=0.016），而线粒体超氧化物水平、DNA 损伤水平和克隆形成存活能力不受影响。总之，我们发现静态和 50Hz MFs 可能会改变对阿霉素治疗的反应，但两种类型的 MFs 对阿霉素处理后的癌细胞的后续存活没有影响。因此，本研究结果表明，3 小时的静态或 50Hz MFs 不会改变 MCF-7 癌细胞中阿霉素的疗效。
