Department of Agronomy, Hetao College, Bayannur, Inner Mongolia 015000, China.
Bayannur Forestry and Grassland Bureau, Bayannur Inner Mongolia 015000, China.
Scanning. 2022 Jun 16;2022:3323768. doi: 10.1155/2022/3323768. eCollection 2022.
In order to explore the practical application of ultrasonic imaging in the pregnancy stage of Mongolian sheep and the role of L-selectin in the embryo implantation process of Mongolian sheep, this paper systematically observed the early embryonic development by B-mode ultrasonic imaging wave diagnostic instrument with 5 MHz rectal probe and detected the expression of sLex and L-selectin in embryonic cells (jar cells) and endometrial cells (RL95-2 cells) by immunoassay to show the role of L-selectin in embryonic adhesion. The results were as follows: the correct rate of fetal sex determination by ultrasound imaging increased with the increase of pregnancy days and reached 93% at 84 days; sLex/L-selectin on the surface of Jar/RL95-2 cells is involved in the adhesion between embryo and endometrium; and when the concentration of L-selectin was 30 g/ml, the implantation success rate of fertilized eggs and embryos was the highest, reaching 95%. It is proved that ultrasonic intelligent imaging exploration can summarize the imaging characteristics of the early development law of sheep fetus, which provides a basis for B-ultrasound to monitor fetal growth and predict fetal age. While discussing the molecular mechanism of implantation, it provides a new idea and means for the clinical intervention of contraception and pregnancy assistance with oligosaccharide as the target.
为探索超声成像技术在蒙古绵羊妊娠阶段的实际应用及 L-选择素在绵羊胚胎着床过程中的作用，本研究采用 5MHz 直肠探头的 B 型超声成像波诊断仪系统观察早期胚胎发育情况，并通过免疫检测法检测胚胎细胞（Jar 细胞）和子宫内膜细胞（RL95-2 细胞）中 sLex 和 L-选择素的表达，以显示 L-选择素在胚胎黏附中的作用。结果如下：超声成像法鉴定胎儿性别的准确率随妊娠天数的增加而提高，在 84 天时达到 93%；Jar/RL95-2 细胞表面的 sLex/L-选择素参与了胚胎与子宫内膜的黏附；当 L-选择素浓度为 30μg/ml 时，受精卵和胚胎的着床成功率最高，达到 95%。证明超声智能成像探测可以总结绵羊胎儿早期发育规律的成像特征，为 B 超监测胎儿生长和预测胎儿年龄提供了依据。在探讨着床的分子机制时，为以寡糖为靶点的避孕和妊娠辅助的临床干预提供了新的思路和手段。
