Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Japan.
Arq Gastroenterol. 2022 Apr-Jun;59(2):288-295. doi: 10.1590/S0004-2803.202202000-51.
Polysaccharides from edible mushrooms possess immunomodulatory, anti-inflammatory, and anti-tumor activities. Recent studies indicated that necroptosis plays a role in the pathogenesis of inflammatory diseases and mediates increased expression of inflammatory cytokines.
Therefore, it is imperative to determine the impact of polysaccharide extract from Lentinula edodes (L. edodes) on inflammatory cytokines in experimental model of colitis in mice.
Female C57BL/6 mice divided into three or four mice per group were used for this study. Polysaccharide sample was orally administered to mice prior to (7 days) and during colitis induction with 2.5% dextran sodium sulfate (7 days), followed by additional 3 days of administration. Changes in body weight and colon length were used as markers for colitis, and pro-inflammatory cytokines and tumor necrosis factor receptor 1 (TNFR1) expressions, as well as necroptosis were analyzed in the colon of colitis mice. Data obtained were analysed by Tukey-Kramer and two-tailed standard t tests.
The results indicated that the polysaccharide sample suppressed colitis in mice using effects on the body weight and colon length as markers. Also, it was demonstrated that necrostatin-1, a specific inhibitor of necroptosis, suppressed the expression of interleukin (IL)-8, a pro-inflammatory chemokine, in Caco-2 cells induced necroptosis induced by zVAD and TNF-α, an indication that necroptosis may be involved in the expression of pro-inflammatory cytokines. Moreover, the polysaccharide sample suppressed the expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, IL-6, IL-1β, and interferon (IFN)-γ in the colon of mice.
These results suggested that the suppressive effects of the polysaccharide sample on inflammatory cytokines expression may contribute to its anti-colitis effect, and so may serve as a potent therapeutic agent against inflammatory bowel disease.
食用蘑菇中的多糖具有免疫调节、抗炎和抗肿瘤活性。最近的研究表明，细胞坏死性凋亡在炎症性疾病的发病机制中起作用，并介导炎症细胞因子的表达增加。
因此，确定香菇多糖提取物（香菇）对实验性结肠炎模型中小鼠炎症细胞因子的影响是当务之急。
本研究使用雌性 C57BL/6 小鼠，每组 3 或 4 只。多糖样品在结肠炎诱导前（7 天）和诱导期间（7 天，用 2.5%葡聚糖硫酸钠）口服给予小鼠，然后再给予 3 天。体重和结肠长度的变化被用作结肠炎的标志物，并且在结肠炎小鼠的结肠中分析促炎细胞因子和肿瘤坏死因子受体 1（TNFR1）的表达以及细胞坏死性凋亡。通过 Tukey-Kramer 和双尾标准 t 检验分析获得的数据。
结果表明，多糖样品通过对体重和结肠长度的影响来抑制结肠炎。此外，还表明细胞坏死性凋亡特异性抑制剂 necrostatin-1 抑制了由 zVAD 和 TNF-α诱导的 Caco-2 细胞中细胞坏死性凋亡诱导的促炎趋化因子白细胞介素（IL）-8 的表达，这表明细胞坏死性凋亡可能参与了促炎细胞因子的表达。此外，多糖样品抑制了 TNF-α、IL-6、IL-1β和干扰素（IFN）-γ等促炎细胞因子在结肠炎小鼠结肠中的表达。
这些结果表明，多糖样品对炎症细胞因子表达的抑制作用可能有助于其抗结肠炎作用，因此可能成为治疗炎症性肠病的有效药物。
