Universidade Federal de Pelotas - UFPel, Pelotas Dental School, Pelotas, RS, Brazil.
University of North Carolina - UNC, Adams School of Dentistry, Division of Comprehensive Oral Health, Chapel Hill, NC, United States.
Braz Oral Res. 2022 Jul 11;36:e091. doi: 10.1590/1807-3107bor-2022.vol36.0091. eCollection 2022.
The objective of this study was to formulate an experimental light-cured periodontal dressing containing alpha-humulene and to compare its physical, antimicrobial, and cytotoxicity properties with commercial gold standards (Barricaid® and Periobond®). Two periodontal dressing formulations were developed (a and b). The formulations were divided into 5 groups according to the alpha-humulene concentration as follows: Ea - control group, Ea1 - 1%, Ea5 - 5%, Ea10 - 10%, and Ea20 - 20%; Eb - control group, Eb1 - 1%, Eb5 - 5%, Eb10 - 10%, and Eb20 - 20%. Materials characterization was performed using the degree of conversion, cohesive strength, sorption, and solubility assays. Antimicrobial assay was performed using the modified direct contact test against E. faecalis and S. aureus. Cytotoxicity was assessed by the cell viability experiment using L929 fibroblasts. In general, the cohesive strength values of materials decreased as the alpha-humulene concentration increased. All the experimental dressings showed antimicrobial activity against both bacteria tested. Cell viability results for the Ea, Ea1, Eb, and Eb1 groups showed moderate cytotoxic effect. The formulations containing alpha-humulene showed similar behavior to the commercial references. Thus, formulations containing alpha-humulene have potential to be used as periodontal dressing.
本研究的目的是制备一种含有葎草烯的实验性光固化牙周敷料，并将其物理性能、抗菌性能和细胞毒性与商业金标准（Barricaid®和 Periobond®）进行比较。制备了两种牙周敷料配方（a 和 b）。根据葎草烯的浓度，将配方分为 5 组，如下所示：Ea-对照组，Ea1-1%，Ea5-5%，Ea10-10%，Ea20-20%；Eb-对照组，Eb1-1%，Eb5-5%，Eb10-10%，Eb20-20%。使用转化率、内聚强度、吸附和溶解度测定来进行材料特性分析。采用改良的直接接触试验对粪肠球菌和金黄色葡萄球菌进行抗菌试验。通过 L929 成纤维细胞的细胞活力实验评估细胞毒性。通常，随着葎草烯浓度的增加，材料的内聚强度值降低。所有实验性敷料均显示出对两种测试细菌的抗菌活性。Ea、Ea1、Eb 和 Eb1 组的细胞活力结果显示出中度细胞毒性作用。含有葎草烯的配方表现出与商业参考物相似的行为。因此，含有葎草烯的配方具有作为牙周敷料的潜力。
