Department of Microbiology, DAV University, Jalandhar, Punjab, India.
Department of Biotechnology, DAV University, Jalandhar, Punjab, India.
Mol Cell Biochem. 2024 Feb;479(2):283-296. doi: 10.1007/s11010-023-04729-9. Epub 2023 Apr 15.
The purpose of this study was to determine whether or not there were significant differences in the antibacterial potential of Thuja occidentalis collected from four distinct geographical sites, namely Chamba (Himachal Pradesh, India), Jalandhar (Punjab, India), Aurangabad (Bihar, India) and Kakching (Manipur, India). The plant extracts were prepared in three different solvents: ethanol, methanol, and acetone. The antibacterial potential of the plant extracts was tested against five different bacterial species using well diffusion test. The minimum inhibitory and bactericidal concentrations of the plant sample exhibiting maximum zone of inhibition against different bacterial strains were calculated. Further, the total phenols, flavonoids, and antioxidant efficacy (using DPPH assay) were also analysed biochemically. The activity of different antioxidant enzymes including SOD, CAT and APX were also recorded as these enzymes protect the cells from free radical damage. GC-MS analysis was also performed on all plant extracts to identify the bioactive components. The results showed that the T. occidentalis collected from the Kakching, Manipur, East side of India showed the highest zone of inhibition against all the bacterial strains, followed by Chamba, Jalandhar, and lastly Aurangabad. To analyse the impact of phytochemicals on the antibacterial efficacy, a correlation was drawn between the biochemical parameters and zone of inhibition using Karl Pearson's method. Most bacterial species demonstrated a positive correlation between antibacterial effectiveness (zone of inhibition) and biochemical markers. The GC-MS study revealed positive correlation between zone of inhibition and peak area percentages of α-Pinene, β-caryophyllene, Germacrene-D, and Humulene in all bacterial species indicating that these chemicals may play a key role in the bactericidal potential of T. occidentalis. Based on the results of this investigation, it is evident that the antibacterial effectiveness of T. occidentalis varies with its geographical location which may be attributed to the differences in the phytochemical makeup.
本研究旨在确定从四个不同地理位置（印度喜马偕尔邦的昌巴、印度旁遮普邦的贾朗达尔、印度比哈尔邦的奥朗加巴德和印度曼尼普尔邦的卡钦）采集的西洋杉的抗菌潜力是否存在显著差异。将植物提取物用三种不同溶剂（乙醇、甲醇和丙酮）制备。采用平板扩散法测定植物提取物对五种不同细菌的抗菌潜力。计算对不同细菌菌株表现出最大抑菌圈的植物样品的最小抑菌浓度和杀菌浓度。此外，还通过 DPPH 测定法进行了总酚、类黄酮和抗氧化活性（使用 DPPH 测定法）的生化分析。还记录了不同抗氧化酶（包括 SOD、CAT 和 APX）的活性，因为这些酶可以保护细胞免受自由基的损伤。对所有植物提取物进行了 GC-MS 分析，以鉴定生物活性成分。结果表明，从印度曼尼普尔邦卡钦地区采集的西洋杉对所有细菌菌株的抑制圈最大，其次是印度喜马偕尔邦的昌巴、印度旁遮普邦的贾朗达尔，最后是印度比哈尔邦的奥朗加巴德。为了分析植物化学物质对抗菌功效的影响，使用 Karl Pearson 方法绘制了生化参数与抑菌圈之间的相关性。大多数细菌菌株的抗菌效果（抑菌圈）与生化标志物之间呈正相关。GC-MS 研究表明，在所有细菌中，抑菌圈与α-蒎烯、β-石竹烯、大根香叶烯 D 和葎草烯的峰面积百分比之间存在正相关，这表明这些化学物质可能在西洋杉的杀菌潜力中发挥关键作用。基于本研究的结果，显然西洋杉的抗菌效果因地理位置而异，这可能归因于其化学成分的差异。
