CRISPR Del/Rei：一种无疤痕基因组编辑的简单、灵活、高效的流水线。
CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing.
机构信息
McKusick-Nathans Department of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, USA.
Predoctoral Training Program in Human Genetics and Molecular Biology, Johns Hopkins School of Medicine, Baltimore, USA.
出版信息
Sci Rep. 2022 Jul 13;12(1):11928. doi: 10.1038/s41598-022-16004-w.
Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.
无痕编辑诱导多能干细胞(iPSC)的基因组对于精确建模遗传疾病至关重要。在这里，我们提出了 CRISPR Del/Rei，这是一种具有高效编辑效率和简单基于 PCR 的筛选的两步缺失-插入策略，可在大约 2 个月内生成同基因克隆。我们应用我们的策略在 3 个基因座上编辑 iPSC，仅发生罕见的脱靶编辑。
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