Centro de Ciências Biológicas e da Saúde, Universidade do Vale do Taquari - UNIVATES, Av. Avelino Tallini, 171, Bairro Universitário, CEP 95914-014, Lajeado, RS, Brasil.
Centro de Ciências Exatas e Tecnológicas, Universidade do Vale do Taquari - UNIVATES, Av. Avelino Tallini, 171, Bairro Universitário, CEP 95914-014, Lajeado, RS, Brasil.
Braz J Biol. 2021 Jan-Feb;81(1):69-76. doi: 10.1590/1519-6984.219889.
The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS. The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS. The essential oils were obtained by hydrodistillation in a modified Clevenger apparatus. The yields were 4.44% in April, and 6.15%, in October. Thirty-one compounds were identified in EOA, accounting for 96.87% of the total, and twenty-eight compounds were identified in EOO, accounting for 99.46% of the total. For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was α-cadinol (10.72%), while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%), whereas the major identified sesquiterpene was α-cadinol (12.79%). The antibacterial activity of the essential oil was evaluated against the three Listeria monocytogenes from the American Type Culture Collection (strains ATCC 7644, 19114 and 13932). The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932. Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL.
采用 GC-MS 对金橘（Campomanesia aurea）叶的精油（EO）成分进行了分析。该植物于四月（EOA）和十月（EOO）在阿雷格里港/南里奥格兰德州采集。精油通过改良的 Clevenger 装置进行水蒸馏提取。四月的产率为 4.44%，十月的产率为 6.15%。在 EOA 中鉴定出 31 种化合物，占总含量的 96.87%，在 EOO 中鉴定出 28 种化合物，占总含量的 99.46%。对于 EOA，主要鉴定的单萜是对伞花烃（8.33%），主要鉴定的倍半萜是α-杜松醇（10.72%），而对于 EOO，主要鉴定的单萜是重复的松油烯-4-醇（3.43%），主要鉴定的倍半萜是α-杜松醇（12.79%）。评估了精油对三种来自美国模式培养物集存库（菌株 ATCC 7644、19114 和 13932）的单核细胞增生李斯特菌的抗菌活性。精油对 ATCC 7644 和 19114 菌株的 MIC 为 10 mg/mL，对 20 mg/mL 具有抑菌作用，对 ATCC 13932 的 MIC 为 20 mg/mL。关于抗生物膜活性，EOO 表现出最好的结果，在应用浓度为 4.0 mg/mL 时，对固定生长的抑制率为 90%。
