蔓橘化学成分分析及其对三种单核细胞增生李斯特菌的抗菌活性研究

Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes.

机构信息

Centro de Ciências Biológicas e da Saúde, Universidade do Vale do Taquari - UNIVATES, Av. Avelino Tallini, 171, Bairro Universitário, CEP 95914-014, Lajeado, RS, Brasil.

Centro de Ciências Exatas e Tecnológicas, Universidade do Vale do Taquari - UNIVATES, Av. Avelino Tallini, 171, Bairro Universitário, CEP 95914-014, Lajeado, RS, Brasil.

出版信息

Braz J Biol. 2021 Jan-Feb;81(1):69-76. doi: 10.1590/1519-6984.219889.

DOI:10.1590/1519-6984.219889

PMID:32130285

Abstract

The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS. The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS. The essential oils were obtained by hydrodistillation in a modified Clevenger apparatus. The yields were 4.44% in April, and 6.15%, in October. Thirty-one compounds were identified in EOA, accounting for 96.87% of the total, and twenty-eight compounds were identified in EOO, accounting for 99.46% of the total. For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was α-cadinol (10.72%), while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%), whereas the major identified sesquiterpene was α-cadinol (12.79%). The antibacterial activity of the essential oil was evaluated against the three Listeria monocytogenes from the American Type Culture Collection (strains ATCC 7644, 19114 and 13932). The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932. Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL.

摘要

采用 GC-MS 对金橘（Campomanesia aurea）叶的精油（EO）成分进行了分析。该植物于四月（EOA）和十月（EOO）在阿雷格里港/南里奥格兰德州采集。精油通过改良的 Clevenger 装置进行水蒸馏提取。四月的产率为 4.44%，十月的产率为 6.15%。在 EOA 中鉴定出 31 种化合物，占总含量的 96.87%，在 EOO 中鉴定出 28 种化合物，占总含量的 99.46%。对于 EOA，主要鉴定的单萜是对伞花烃（8.33%），主要鉴定的倍半萜是α-杜松醇（10.72%），而对于 EOO，主要鉴定的单萜是重复的松油烯-4-醇（3.43%），主要鉴定的倍半萜是α-杜松醇（12.79%）。评估了精油对三种来自美国模式培养物集存库（菌株 ATCC 7644、19114 和 13932）的单核细胞增生李斯特菌的抗菌活性。精油对 ATCC 7644 和 19114 菌株的 MIC 为 10 mg/mL，对 20 mg/mL 具有抑菌作用，对 ATCC 13932 的 MIC 为 20 mg/mL。关于抗生物膜活性，EOO 表现出最好的结果，在应用浓度为 4.0 mg/mL 时，对固定生长的抑制率为 90%。