Chen Xiushuang, An Miao, Zhang Wenqian, Li Kun, Kulyar Muhammad Fakhar-E-Alam, Duan Kun, Zhou Hui, Wu Yu, Wan Xin, Li Jianlong, Quan Lingtong, Mai Zhanhai, Bai Wenxia, Wu Yi
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Front Vet Sci. 2022 Jun 27;9:918541. doi: 10.3389/fvets.2022.918541. eCollection 2022.
The gut microbial community is closely related to mastitis, but studies regarding the influences of mastitis on gut microbiota in buffalo remain scarce. Herein, we characterized the differences in gut bacterial and fungal communities between mastitis-affected and healthy buffalos. Interestingly, although mastitis had no effect on gut bacterial and fungal diversities in the buffalos, some bacterial and fungal taxa were significantly altered. Bacterial and fungal taxonomic analysis showed that the preponderant bacterial phyla (Firmicutes and Bacteroidetes) and fungal phyla (Ascomycota and Basidiomycota) in buffalo were the same regardless of health status. At the level of genus, the changes in some gut bacterial and fungal abundances between both groups were gradually observed. Compared with healthy buffalos, the proportions of 3 bacterial genera (, and ) and 1 fungal genus () in the mastitis-affected buffalo were significantly increased, whereas 4 bacterial genera (, and ) and 4 fungal genera ( and ) were significantly decreased. Taken together, this research revealed that there was significant difference in the compositions of the gut microbial community between the healthy and mastitis-affected buffalos. To our knowledge, this is the first insight into the characteristics of the gut microbiota in buffalos with mastitis, which is beneficial to understand the gut microbial information of buffalo in different health states and elucidate the pathogenesis of mastitis from the gut microbial perspective.
肠道微生物群落与乳腺炎密切相关，但关于乳腺炎对水牛肠道微生物群影响的研究仍然很少。在此，我们表征了患乳腺炎的水牛和健康水牛之间肠道细菌和真菌群落的差异。有趣的是，尽管乳腺炎对水牛的肠道细菌和真菌多样性没有影响，但一些细菌和真菌类群发生了显著变化。细菌和真菌分类分析表明，无论健康状况如何，水牛中占优势的细菌门（厚壁菌门和拟杆菌门）和真菌门（子囊菌门和担子菌门）都是相同的。在属水平上，逐渐观察到两组之间一些肠道细菌和真菌丰度的变化。与健康水牛相比，患乳腺炎的水牛中3个细菌属（、和）和1个真菌属（）的比例显著增加，而4个细菌属（、和）和4个真菌属（和）显著减少。综上所述，本研究揭示了健康水牛和患乳腺炎的水牛之间肠道微生物群落组成存在显著差异。据我们所知，这是对患乳腺炎水牛肠道微生物群特征的首次深入了解，这有助于了解不同健康状态下水牛的肠道微生物信息，并从肠道微生物角度阐明乳腺炎的发病机制。
