Gong Chaihong, Mao Xiaowei, Wang Zhe, Luo Zhang, Liu Zhifan, Ben Yali, Zhang Weiying, Guo Zhenzhong
School of Life Science, Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan, China.
School of Environment and Health, Jianghan University, Wuhan, China.
Front Bioeng Biotechnol. 2022 Jun 22;10:891727. doi: 10.3389/fbioe.2022.891727. eCollection 2022.
Despite recent progress, a challenge remains on how to gently release and recover viable ctDNA captured on DNA probe-based devices. Here, a reusable detector was successfully manufactured for the capture and release of ctDNA by means of an UCNPs@SiO-Azo/CD-probe. Biocompatible NIR light is used to excite UCNPs and convert into local UV light. Continuous irradiation induces a rapid release of the entire ctDNA-probe-CD complex from the functionalized surface the trans-cis isomerization of azo units without disrupting the ctDNA-structure receptor. Specifically, these composite chips allow reloading DNA probes for reusable ctDNA detection with no obvious influence on their efficiency. The results of our study demonstrated the potential application of this platform for the quantitative detection of ctDNA and the individualized analysis of cancer patients.
尽管最近取得了进展，但在如何温和地释放和回收基于DNA探针的设备上捕获的可存活循环肿瘤DNA（ctDNA）方面仍然存在挑战。在此，通过上转换纳米粒子@二氧化硅-偶氮/环糊精探针成功制造了一种可重复使用的探测器，用于捕获和释放ctDNA。生物相容性近红外光用于激发上转换纳米粒子并转化为局部紫外光。连续照射会使整个ctDNA-探针-环糊精复合物从功能化表面快速释放，这是由于偶氮单元的反式-顺式异构化，而不会破坏ctDNA结构受体。具体而言，这些复合芯片允许重新加载DNA探针以进行可重复使用的ctDNA检测，且对其效率没有明显影响。我们的研究结果证明了该平台在ctDNA定量检测和癌症患者个体化分析中的潜在应用。
