Yu Haiyan, Tang Donge, Wu Hongwei, Li Chunhong, Lu Yongping, He Fang, Zhang Xiaogang, Yang Yane, Shi Wei, Hu Wenlong, Zeng Zhipeng, Dai Weier, Ou Minglin, Dai Yong
Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
Department of Pharmacy, Shenzhen Pingshan District People's Hospital, Pingshan General Hospital of Southern Medical University, Shenzhen, Guangdong 518118, P.R. China.
iScience. 2022 Jun 27;25(7):104679. doi: 10.1016/j.isci.2022.104679. eCollection 2022 Jul 15.
The spine has essential roles in supporting body weight, and passaging the neural elements between the body and the brain. In this study, we used integrated single-cell RNA sequencing and single-cell transposase-accessible chromatin sequencing analyses to reveal the cellular heterogeneity, lineage, and transcriptional regulatory network of the developing human spine. We found that + + fibroblasts with stem cell characteristics could differentiate into chondrocytes by highly expressing the chondrogenic markers and . Neurons could originate from neuroendocrine cells, and MEIS2 may be an essential transcription factor that promotes spinal neural progenitor cells to selectively differentiate into neurons during early gestation. Furthermore, the interaction of NRP2_SEMA3C and CD74_APP between macrophages and neurons may be essential for spinal cord development. Our integrated map provides a blueprint for understanding human spine development in the early and midgestational stages at single-cell resolution and offers a tool for investigating related diseases.
脊柱在支撑体重以及在身体与大脑之间传递神经元件方面发挥着重要作用。在本研究中，我们使用整合的单细胞RNA测序和单细胞转座酶可及染色质测序分析，以揭示发育中的人类脊柱的细胞异质性、谱系和转录调控网络。我们发现具有干细胞特征的双阳性成纤维细胞可通过高表达软骨生成标志物和而分化为软骨细胞。神经元可能起源于神经内分泌细胞，并且MEIS2可能是在妊娠早期促进脊髓神经祖细胞选择性分化为神经元的关键转录因子。此外，巨噬细胞与神经元之间的NRP2_SEMA3C和CD74_APP的相互作用可能对脊髓发育至关重要。我们的整合图谱提供了一个蓝图，用于在单细胞分辨率下理解妊娠早期和中期的人类脊柱发育，并为研究相关疾病提供了一个工具。
