Liu Danling, Zhang Yunzhong, Guo Luo, Fang Rui, Guo Jin, Li Peifang, Qian Tingting, Li Wen, Zhao Liping, Luo Xiaoning, Zhang Siyi, Shao Jun, Sun Shan
Department of Otorhinolaryngology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Guangdong Cardiovascular Institute, Southern Medical University, Guangzhou, China.
ENT Institute and Otorhinolaryngology, Innovation Center, Affiliated Eye and ENT Hospital, Key Laboratory of Hearing Medicine of NHFPC, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.
Cell Prolif. 2024 Dec;57(12):e13723. doi: 10.1111/cpr.13723. Epub 2024 Sep 8.
The vocal fold is an architecturally complex organ comprising a heterogeneous mixture of various layers of individual epithelial and mesenchymal cell lineages. Here we performed single-cell RNA sequencing profiling of 5836 cells from the vocal folds of adult Sprague-Dawley rats. Combined with immunostaining, we generated a spatial and transcriptional map of the vocal fold cells and characterized the subpopulations of epithelial cells, mesenchymal cells, endothelial cells, and immune cells. We also identified a novel epithelial-to-mesenchymal transition-associated epithelial cell subset that was mainly found in the basal epithelial layers. We further confirmed that this subset acts as intermediate cells with similar genetic features to epithelial-to-mesenchymal transition in head and neck squamous cell carcinoma. Finally, we present the complex intracellular communication network involved homeostasis using CellChat analysis. These studies define the cellular and molecular framework of the biology and pathology of the VF mucosa and reveal the functional importance of developmental pathways in pathological states in cancer.
声带是一个结构复杂的器官，由不同上皮和间充质细胞谱系的各层组成的异质混合物构成。在这里，我们对成年Sprague-Dawley大鼠声带的5836个细胞进行了单细胞RNA测序分析。结合免疫染色，我们生成了声带细胞的空间和转录图谱，并对上皮细胞、间充质细胞、内皮细胞和免疫细胞的亚群进行了表征。我们还鉴定出一种新型的上皮-间充质转化相关上皮细胞亚群，主要存在于基底上皮层。我们进一步证实，该亚群作为中间细胞，具有与头颈部鳞状细胞癌上皮-间充质转化相似的遗传特征。最后，我们使用CellChat分析展示了参与稳态的复杂细胞内通讯网络。这些研究定义了声带黏膜生物学和病理学的细胞和分子框架，并揭示了发育途径在癌症病理状态中的功能重要性。
