miR-197、miR-26a 和 miR-27a 在慢性淋巴细胞白血病中的分析。

miR-197, miR-26a and miR-27a analysis in chronic lymphocytic leukemia.

机构信息

Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, 6627, Presidente Antônio Carlos Ave, Pampulha, Belo Horizonte, MG, 31270901, Brazil.

Clinical Hospital, Federal University of Minas Gerais, Presidente Antônio Carlos Ave, Pampulha, Belo Horizonte, MG, 31270901, Brazil.

出版信息

Biomark Med. 2022 Aug;16(12):903-914. doi: 10.2217/bmm-2021-0873. Epub 2022 Jul 14.

DOI:10.2217/bmm-2021-0873

PMID:35833845

Abstract

Chronic lymphocytic leukemia (CLL) involves the proliferation and increase of B-lymphocytes in the peripheral blood, bone marrow and lymphoid organs. This study evaluated the microRNAs miR-197, miR-26a and miR-27a as potential biomarkers for CLL. Eighty-two patients with CLL and 62 control subjects (CT) were investigated for these targets, using quantitative PCR (qPCR). A significant reduction of all microRNAs was observed in CLL compared to the controls (p < 0.001). Significant negative correlations were observed for the clinical staging groups. After adjusting for multiple logistic regression analysis, miR-197 and miR-26a remained as possible independent risk factors related to the CLL. Our data indicated good performance of this microRNAs as potential biomarkers in CLL.

摘要

慢性淋巴细胞白血病（CLL）涉及外周血、骨髓和淋巴器官中 B 淋巴细胞的增殖和增加。本研究评估了 microRNAs miR-197、miR-26a 和 miR-27a 作为 CLL 的潜在生物标志物。使用定量 PCR（qPCR）对 82 例 CLL 患者和 62 例对照（CT）进行了这些靶标检测。与对照组相比，CLL 中所有 microRNAs 的表达均显著降低（p<0.001）。临床分期组之间观察到显著的负相关。在进行多元逻辑回归分析调整后，miR-197 和 miR-26a 仍然是与 CLL 相关的可能独立危险因素。我们的数据表明，这些 microRNAs 作为 CLL 的潜在生物标志物具有良好的性能。