Institute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guiyang 550025, Guizhou, P. R. China.
Environ Entomol. 2022 Aug 19;51(4):780-789. doi: 10.1093/ee/nvac053.
The western flower thrip (WFT) Frankliniella occidentalis (Pergande) is a serious agricultural pest with a wide host range which has developed resistance to several groups of insecticides. In this study, the effect of insecticide resistance on WFT host adaptability was explored by examining changes in detoxification enzyme activities and thrip development, and reproduction on preferred and less preferred host plants, eggplant Solanum melongena L. and broad bean Vicia faba L., respectively. Thrips were screened with spinetoram on kidney bean for six generations. Activities of glutathione S-transferase (GST), mixed function oxidases (MFOs), and cytochrome P450 enzyme (P450) in a resistant strain (RS) reared on broad bean were significantly higher than those in a sensitive strain (SS), and only carboxylesterase (CarE) increased in the RS when reared on eggplant, compared with the SS. Activities of the four detoxification enzymes in the RS reared on broad bean were significantly higher than in those reared-on eggplant. On broad bean, RS adult longevity was lower and developmental duration of offspring was shorter than those of the SS, but fecundity increased. On eggplant, RS fecundity was lower and developmental duration of offspring was shorter than those of the SS. In addition, fecundity was higher and developmental duration was longer in the RS reared on broad bean than in those reared-on eggplant. The results indicated that spinetoram resistance could change WFT host preference and that those changes might be associated with detoxification enzyme activities. Thus, it was hypothesized that adaptability of the RS to the less preferred host broad bean increased, whereas adaptability to the preferred host eggplant decreased.
西方花蓟马（WFT）Frankliniella occidentalis（Pergande）是一种严重的农业害虫，具有广泛的宿主范围，已对几类杀虫剂产生了抗性。在这项研究中，通过研究解毒酶活性的变化以及在首选和较不喜欢的宿主植物（茄子 Solanum melongena L. 和蚕豆 Vicia faba L.）上的发育和繁殖，探讨了杀虫剂抗性对 WFT 宿主适应性的影响。用螺虫乙酯对豇豆进行了六代筛选。在蚕豆上饲养的抗性品系（RS）的谷胱甘肽 S-转移酶（GST）、多功能氧化酶（MFO）和细胞色素 P450 酶（P450）的活性明显高于敏感品系（SS），而在茄子上饲养时，只有羧酸酯酶（CarE）在 RS 中增加，与 SS 相比。在蚕豆上饲养的 RS 的四种解毒酶活性明显高于在茄子上饲养的 RS。在蚕豆上，RS 成虫寿命较低，后代发育时间较短，但繁殖力增加。在茄子上，RS 的繁殖力较低，后代的发育时间较短。此外，在蚕豆上饲养的 RS 的繁殖力高于在茄子上饲养的 RS。结果表明，螺虫乙酯抗性可以改变 WFT 的宿主偏好，并且这些变化可能与解毒酶活性有关。因此，假设 RS 对较不喜欢的宿主蚕豆的适应性增加，而对首选宿主茄子的适应性降低。
