从固态发酵的扎辣椒中分离到两株新型戊糖发酵和 GABA 产生菌：图伊氏乳杆菌新种和天使副拟杆菌新种。

Characterization of two novel pentose-fermenting and GABA-producing species: Levilactobacillus tujiorum sp. nov. and Secundilactobacillus angelensis sp. nov. Isolated from a solid-state fermented zha-chili.

机构信息

Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang, Hubei, PR China; Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory, Hubei University of Arts and Science, Xiangyang, Hubei, PR China.

Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang, Hubei, PR China; Hubei Key Laboratory of Yeast Function, Yichang, Hubei, PR China.

出版信息

Syst Appl Microbiol. 2022 Sep;45(5):126344. doi: 10.1016/j.syapm.2022.126344. Epub 2022 Jul 4.

DOI:10.1016/j.syapm.2022.126344

PMID:35834933

Abstract

Lactobacilli are dominant in zha-chili. This study provides a taxonomic characterization of five bacterial strains isolated from zha-chili in China. The cells were Gram-positive, facultative anaerobic, non-spore-forming, flagella-free, catalase-negative, heterofermentative, pentose-fermenting, and gamma-aminobutyric acid (GABA)-producing rods. For HBUAS51241, HBUAS51329, and HBUAS51416, C, Cω9c and C iso were the predominant cellular fatty acids; diphosphatidylglycerol (DPG), phosphatidylglycerol (DP), glycolipids (GL), and glycolipids (AL) were the major phospholipids. While for HBUAS51383 and HBUAS58055, C, Cω9c, C cyclo ω8c were the predominant cellular fatty acids; DPG, DP, GL, and AL were the major phospholipids. Strains HBUAS51241, HBUAS51329, and HBUAS51416 showed 98.1-99.1% 16S rRNA gene sequence similarity, 80.2-81.4% ANI, 87.7-90.0% AAI, and 23.8-32.8% digital DDH to their closest related type strains Levilactobacillus hammesii DSM 16381, Levilactobacillus parabrevis ATCC 53295, and Levilactobacillus fuyuanensis 244-4. Strains HBUAS51383 and HBUAS58055 showed 98.7-99.5% 16S rRNA gene sequence similarity, 75.4-81.4% ANI, 75.5-89.1% AAI, and 19.7-24.0% digital DDH to their closest related type strains Secundilactobacillus silagincola IWT5, Secundilactobacillus silagei JCM 19001, Secundilactobacillus pentosiphilus IWT25, Secundilactobacillus mixtipabuli IWT30, Secundilactobacillus odoratitofui DSM 19909, and Secundilactobacillus similis DSM 23365. The central carbon metabolism pathways for the five strains were summarizeded. Based on the phenotypic, chemotaxonomic, and genomic data, we propose two novel species Levilactobacillus tujiorum sp. nov. whose type strain is HBUAS51241 (=GDMCC 1.3022 = JCM 35241), and Secundilactobacillus angelensis sp. nov. whose type strain is HBUAS51383 (=GDMCC 1.3021 = JCM 35209).

摘要

植物乳杆菌是扎辣椒中的优势菌。本研究对从中国扎辣椒中分离得到的 5 株细菌菌株进行了分类学特征描述。这些细胞呈革兰氏阳性、兼性厌氧、不产芽孢、无鞭毛、过氧化氢酶阴性、异型发酵、戊糖发酵和产生γ-氨基丁酸（GABA）。对于 HBUAS51241、HBUAS51329 和 HBUAS51416，C、Cω9c 和 Ciso 是主要的细胞脂肪酸；双磷脂酰甘油（DPG）、磷脂酰甘油（DP）、糖脂（GL）和糖脂（AL）是主要的磷脂。而对于 HBUAS51383 和 HBUAS58055，C、Cω9c、Ccyclo ω8c 是主要的细胞脂肪酸；DPG、DP、GL 和 AL 是主要的磷脂。菌株 HBUAS51241、HBUAS51329 和 HBUAS51416 的 16S rRNA 基因序列相似性为 98.1-99.1%，ANI 为 80.2-81.4%，AAI 为 87.7-90.0%，数字 DDH 为 23.8-32.8%，与最接近的模式菌株 Levilactobacillus hammesii DSM 16381、Levilactobacillus parabrevis ATCC 53295 和 Levilactobacillus fuyuanensis 244-4 相似。菌株 HBUAS51383 和 HBUAS58055 的 16S rRNA 基因序列相似性为 98.7-99.5%，ANI 为 75.4-81.4%，AAI 为 75.5-89.1%，数字 DDH 为 19.7-24.0%，与最接近的模式菌株 Secundilactobacillus silagincola IWT5、Secundilactobacillus silagei JCM 19001、Secundilactobacillus pentosiphilus IWT25、Secundilactobacillus mixtipabuli IWT30、Secundilactobacillus odoratitofui DSM 19909 和 Secundilactobacillus similis DSM 23365 相似。总结了这 5 株菌的中心碳代谢途径。基于表型、化学分类和基因组数据，我们提出了两个新的物种：Levilactobacillus tujiorum sp. nov.，其模式菌株为 HBUAS51241（=GDMCC 1.3022=JCM 35241）；Secundilactobacillus angelensis sp. nov.，其模式菌株为 HBUAS51383（=GDMCC 1.3021=JCM 35209）。