Levilactobacillus yiduensis sp. nov., Isolated from Zha-chili in Yidu County, Hubei Province, China.

The zha-chili isolate HBUAS62285 is a facultative anaerobic strain. This bacterium was gram-positive, but it could not generate catalase, was not motile, did not form spores, had no flagella, and produced gamma-aminobutyric acid (GABA). Comparing of HBUAS62285 with its related type strains Levilactobacillus suantsaiihabitans BCRC 81129, Levilactobacillus angrenensis M1530-1, Levilactobacillus cerevisiae DSM 100836, Levilactobacillus wangkuiensis 6-5(1), Levilactobacillus lanxiensis 13B17, and Levilactobacillus mulengensis 112-3 showed that the similarity of 16S rRNA gene sequence was less than 99.13%. Strain HBUAS62285 has a G+C content of 50.57 mol%, an ANI value of less than 86.61%, an AAI value of less than 92.9%, and a dDDH value of less than 32.9% when compared to the aforementioned closely related strains. In the end, the most major fatty acids in cells were determined to be C, C ω9c, C cyclo ω9,10c, and summed feature 10. Overall, the results of the phenotypic, genomic, chemotaxonomic, and phylogenetic studies indicate that strains HBUAS62285 and CD0817 constitute a new species within the genus Levilactobacillus, and the name Levilactobacillus yiduensis sp. nov. is proposed. The type strain is HBUAS62285 (= JCM 35804 = GDMCC 1.3507).