Key Laboratory of Biodiversity of Aquatic Organisms, Harbin Normal University, Harbin 150025, PR China.
Department of Life Sciences, Natural History Museum, London SW7 5BD, UK.
Eur J Protistol. 2022 Aug;85:125908. doi: 10.1016/j.ejop.2022.125908. Epub 2022 Jul 2.
The morphology and ciliary pattern of two new heterotrich ciliates, Blepharisma orientale n. sp. and B. sinicum n. sp., collected from Sanjiang Plain Wetland, northeastern China, were investigated using living observation and protargol staining. Blepharisma orientale n. sp. is characterized by the following combination of characteristics: limnetic; body in vivo 280-380 × 120-190 μm, slender to elongate, cortical granules dark pink, ventral margin somewhat sigmoid; buccal field about 55-60% of body length; 30-39 somatic kineties; 70-76 adoral membranelles, each comprising two long rows of basal bodies; paroral membrane consists of dikinetids with the posterior third obliquely arranged, both basal bodies in each dikinetid are ciliated. Blepharisma sinicum n. sp. is recognized by the combination of the following characters: limnetic; body 190-260 × 100-170 μm in size in vivo, irregularly sigmoid and slightly bilaterally flattened, dorsal margin curved; cortical granules pale pink in color; buccal field about 60-75% of body length; 25-29 somatic kineties; 53-82 adoral membranelles; paroral membrane consisting entirely of dikinetids. Phylogenetic analyses based on small subunit rRNA (SSU rRNA) gene sequence data recovered the two new species as sister taxa, both the sister group of B. penardi.
两种新的异毛目纤毛虫，东方裸口虫（Blepharisma orientale n. sp.）和中华裸口虫（B. sinicum n. sp.），分别来自中国东北三江平原湿地，采用活体观察和蛋白银染色法对其形态和纤毛模式进行了研究。东方裸口虫的特征如下：生境为淡水；活体大小为 280-380×120-190μm，细长至长形，皮质颗粒深粉红色，腹缘略呈“S”形；口域约占体长的 55-60%；30-39 个体纤毛环；70-76 根口后膜纤毛，每根由两行长的基体组成；膜缘小膜带由联体动基列组成，后三分之一斜向排列，每个联体动基列中的两个基体均具纤毛。中华裸口虫的特征如下：生境为淡水；活体大小为 190-260×100-170μm，形状不规则，略呈双侧扁平，背缘弯曲；皮质颗粒浅粉红色；口域约占体长的 60-75%；25-29 个体纤毛环；53-82 根口后膜纤毛；膜缘小膜带完全由联体动基列组成。基于小亚基 rRNA（SSU rRNA）基因序列数据的系统发育分析结果表明，这两个新种是姐妹种，与 B. penardi 的姐妹群关系最近。
