The European Radiation Synchrotron Facility (ESRF), Grenoble, France.
Bioengineering Department, Northeastern University, Boston, MA, USA.
Curr Opin Struct Biol. 2022 Aug;75:102421. doi: 10.1016/j.sbi.2022.102421. Epub 2022 Jul 11.
Scanning x-ray microdiffraction of complex tissues and materials is an emerging method for the study of macromolecular structures in situ, providing information on the way molecular constituents are arranged and interact with their microenvironment. Acting as a bridge between high-resolution images of individual constituents and lower resolution microscopies that generate global views of material, scanning microdiffraction provides an approach to study the functioning of complex tissues across multiple length scales. Here, we discuss the methodology, summarize results from recent studies, and discuss the potential of the technique for future studies coordinated with other biophysical techniques.
扫描 X 射线微衍射技术是研究复杂组织和材料中大分子结构的新兴方法，提供了关于分子成分排列方式以及与微观环境相互作用的信息。作为个体成分高分辨率图像与生成材料整体视图的低分辨率显微镜之间的桥梁，扫描微衍射提供了一种研究复杂组织在多个长度尺度上功能的方法。本文讨论了该方法学，总结了最近研究的结果，并探讨了该技术与其他生物物理技术相结合进行未来研究的潜力。
