SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
Graduate School of Frontier Biosciences, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.
Anal Chem. 2021 Oct 12;93(40):13520-13526. doi: 10.1021/acs.analchem.1c02396. Epub 2021 Sep 27.
Hemostasis is an essential function that repairs tissues and maintains the survival of living organisms. To prevent diseases caused by the abnormality of the blood coagulation mechanism, it is important to carry out a blood test periodically by a method that is convenient and less burdensome for examiners. Thrombin is a protease that catalyzes the conversion of fibrinogen, and its cleavage activity can be an index of coagulation activity. Here, we developed a ratiometric bioluminescent indicator, Thrombastor (in ctivity ensing indicat), which reflects the thrombin cleavage activity in blood by changing the emission color from green to blue. Compared to the current thrombin activity indicator, the rapid color change of the emission indicated a 2.5-fold decrease in the for thrombin, and the cleavage rate was more than two times faster. By improving the absolute bioluminescence intensity, detection from a small amount of plasma could be achieved with a smartphone camera. Using Thrombastor and a versatile device, an effective diagnosis for preventing coagulation disorders can be provided.
止血是修复组织和维持生物体生存的基本功能。为了预防因凝血机制异常而引起的疾病，定期通过一种对检查者来说方便且负担较小的方法进行血液检查非常重要。凝血酶是一种催化纤维蛋白原转化的蛋白酶，其裂解活性可以作为凝血活性的指标。在这里，我们开发了一种比率型生物发光指示剂，即凝血酶传感器（thrombastor，in ctivity ensing indicat），它通过改变发射颜色从绿色变为蓝色来反映血液中的凝血酶裂解活性。与目前的凝血酶活性指示剂相比，发射的快速颜色变化表明凝血酶的半效浓度降低了 2.5 倍，裂解速度提高了两倍以上。通过提高绝对生物发光强度，我们可以使用智能手机摄像头从少量血浆中进行检测。使用凝血酶传感器和一种多功能设备，可以提供有效的诊断，以预防凝血障碍。
